{"product_id":"pacsin1-antibody-protein-kinase-c-and-casein-kinase-substrate-in-neurons-protein-1-bha17135266","title":"PACSIN1 Antibody \/ Protein kinase C and casein kinase substrate in neurons protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePACSIN1 Antibody \/ Protein kinase C and casein kinase substrate in neurons protein 1 is a anti-PACSIN1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PACSIN1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe PACSIN1 antibody targets Protein kinase C and casein kinase substrate in neurons protein 1, a cytoplasmic protein encoded by the PACSIN1 gene. PACSIN1 belongs to the PACSIN family of F-BAR domain-containing proteins that regulate membrane curvature, endocytosis, and actin cytoskeleton remodeling. Expressed predominantly in neurons, this protein participates in synaptic vesicle recycling and the formation of dendritic spines. The PACSIN1 antibody is a key reagent for exploring how membrane trafficking and cytoskeletal dynamics shape synaptic function and neural connectivity.\u003cbr\u003e\u003cbr\u003eProtein kinase C and casein kinase substrate in neurons protein 1 contains an F-BAR domain that binds curved membranes and an SH3 domain that interacts with dynamin and synaptojanin. Through these interactions, PACSIN1 supports the fission of clathrin-coated vesicles during endocytosis. The PACSIN1 antibody allows detection of this neuronal scaffold protein in both cultured neurons and brain tissues, providing valuable insights into the molecular mechanisms of synaptic vesicle retrieval and recycling.\u003cbr\u003e\u003cbr\u003ePACSIN1 is essential for maintaining proper synaptic transmission and plasticity. Its loss disrupts vesicle endocytosis and actin dynamics, leading to impaired neuronal signaling. The PACSIN1 antibody enables researchers to examine protein expression and distribution across different brain regions, supporting studies of neurotransmission and dendritic spine architecture. It also helps identify post-translational modifications, such as phosphorylation by casein kinase and protein kinase C, that regulate its membrane association and function.\u003cbr\u003e\u003cbr\u003eIn addition to synaptic processes, Protein kinase C and casein kinase substrate in neurons protein 1 has roles in endocytic trafficking in non-neuronal cells and participates in receptor internalization and signaling. Altered expression or mutation of PACSIN1 has been associated with neurological disorders including schizophrenia and neurodevelopmental delay, possibly through disrupted endocytic signaling and synaptic connectivity. The PACSIN1 antibody provides a means to analyze such pathophysiological changes at the protein level.\u003cbr\u003e\u003cbr\u003eExperimental applications for the PACSIN1 antibody include western blotting, immunofluorescence, and immunohistochemistry, where it reveals strong neuronal and synaptic staining.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PACSIN1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PACSIN1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PACSIN1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PACSIN1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PACSIN1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047285547373,"sku":"FY12363","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_3a9dc645-55dd-4679-b30a-40c5b5f9f3be.jpg?v=1782236994","url":"https:\/\/www.ebiohippo.com\/products\/pacsin1-antibody-protein-kinase-c-and-casein-kinase-substrate-in-neurons-protein-1-bha17135266","provider":"BioHippo","version":"1.0","type":"link"}