{"product_id":"pafah1b2-antibody-bha10504179","title":"PAFAH1B2 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis is a polyclonal anti-PAFAH1B2 antibody raised in Rabbit, with confirmed utility in ELISA, IHC, IF. Designed to detect PAFAH1B2 in Human, supporting researchers in cardiovascular contexts.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Recombinant Human Platelet-activating factor acetylhydrolase IB subunit beta protein (2-229AA) — determines the epitope region recognized; confirm epitope compatibility with your sample preparation and expected PTMs.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary reagents. Mismatched secondaries result in no signal. Consider host species when multiplexing.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal:\u003c\/strong\u003e Recognizes multiple epitopes — robust signal across varied preparations, with inherent lot-to-lot variability. Isotype controls and lot qualification recommended.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Secondary antibodies and isotype controls must match IgG from Rabbit. Relevant for multiplexing and Protein A\/G compatibility.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Protein G purification):\u003c\/strong\u003e Reduces non-specific immunoglobulin populations, improving signal-to-noise in quantitative immunoassay formats.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePAFAH1B2\u003c\/strong\u003e (also known as Intracellular platelet activating factor acetylhydrolase alpha 2 subunit, PA1B2_HUMAN, PAF acetylhydrolase 30 kDa subunit, PAF AH 30 kDa subunit, PAF AH beta subunit, PAF AH subunit beta, PAF-AH 30 kDa subunit, PAF-AH subunit beta, PAFAH 30 kDa subunit, PAFAH beta subunit, PAFAH subunit beta, Pafah1b2, PAFAH1B2 protein, PAFAHB, Platelet activating factor acetylhydrolase 1b catalytic subunit 2, Platelet activating factor acetylhydrolase IB beta subunit, Platelet activating factor acetylhydrolase IB subunit beta, Platelet activating factor acetylhydrolase isoform Ib beta subunit, Platelet activating factor acetylhydrolase isoform Ib subunit 2, Platelet-activating factor acetylhydrolase IB subunit beta) is a protein studied in Human systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection outcomes. Researchers are encouraged to consult UniProt, NCBI Gene, and primary literature for current annotation of PAFAH1B2 biology relevant to cardiovascular.\u003c\/p\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Sandwich or indirect ELISA quantifies soluble\/secreted target in biological fluids or cell culture supernatants.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIHC:\u003c\/strong\u003e IHC visualizes target expression in FFPE or frozen sections; optimize antigen retrieval and secondary detection per tissue type.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIF:\u003c\/strong\u003e IF localizes target at subcellular resolution in fixed cells\/sections; fixation and permeabilization conditions affect epitope accessibility.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e Include IgG from Rabbit at equivalent concentration to assess non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity\/isoforms:\u003c\/strong\u003e Polyclonal preparations may cross-react with related proteins or isoforms — validate with siRNA KD, KO lysate, or recombinant protein controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Sample matrix (serum, plasma, lysate, homogenate) may influence performance; pilot dilution linearity and spike-recovery experiments before full quantitative studies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Confirmed for Human; extrapolation to untested species requires empirical validation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\n\n\u003c!-- Sources (internal):\n- UniProt: https:\/\/www.uniprot.org\n- NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n- Cusabio: https:\/\/www.cusabio.com\n- Human Protein Atlas: https:\/\/www.proteinatlas.org\n- PubMed: https:\/\/pubmed.ncbi.nlm.nih.gov\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 ug","offer_id":53238677864813,"sku":"CSB-PA017384LA01HU-50UG","price":170.98,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53238798844269,"sku":"CSB-PA017384LA01HU-100UG","price":307.97,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-PA017384LA01HU-IF.jpg?v=1776672757","url":"https:\/\/www.ebiohippo.com\/products\/pafah1b2-antibody-bha10504179","provider":"BioHippo","version":"1.0","type":"link"}