{"product_id":"pafah1b2-antibody-platelet-activating-factor-acetylhydrolase-ib-subunit-beta-bha17135688","title":"PAFAH1B2 Antibody \/ Platelet-activating factor acetylhydrolase IB subunit beta","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePAFAH1B2 Antibody \/ Platelet-activating factor acetylhydrolase IB subunit beta is a anti-PAFAH1B2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PAFAH1B2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePAFAH1B2 antibody detects Platelet-activating factor acetylhydrolase IB subunit beta, an intracellular enzyme that regulates phospholipid metabolism and brain development. Encoded by the PAFAH1B2 gene on chromosome 11q23.3, this enzyme forms part of the trimeric PAF acetylhydrolase IB complex, which catalyzes the hydrolysis of platelet-activating factor (PAF) and other bioactive phospholipids. The complex consists of one regulatory subunit (LIS1\/PAFAH1B1) and two catalytic subunits (PAFAH1B2 and PAFAH1B3), together maintaining membrane lipid homeostasis and neuronal signaling.\u003cbr\u003e\u003cbr\u003ePAFAH1B2 acts as a cytoplasmic serine hydrolase that removes acetyl groups from PAF, reducing its proinflammatory activity. Beyond lipid metabolism, it contributes to neuronal migration and brain development through its association with LIS1, which regulates microtubule dynamics and dynein-mediated transport. Mutations or dysfunction in components of this complex are associated with lissencephaly and neurodevelopmental defects.\u003cbr\u003e\u003cbr\u003eThe PAFAH1B2 antibody is widely used in neuroscience, lipid signaling, and developmental biology research to investigate lipid hydrolysis, microtubule regulation, and neuronal migration. Western blot analysis identifies a 26 kilodalton band corresponding to PAFAH1B2, while immunofluorescence reveals cytoplasmic localization that overlaps with microtubule structures in neurons. This antibody enables detection of the catalytic subunit in both neuronal and non-neuronal tissues, supporting studies of phospholipid turnover and signaling.\u003cbr\u003e\u003cbr\u003ePAFAH1B2 also plays roles in inflammation and oxidative stress by modulating PAF levels. Abnormal expression has been linked to neuroinflammation and ischemic injury, as well as cancer metabolism where lipid mediators regulate proliferation. The PAFAH1B2 antibody provides a reliable reagent for exploring these processes.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PAFAH1B2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PAFAH1B2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PAFAH1B2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PAFAH1B2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PAFAH1B2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047299834221,"sku":"FY12786","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7929d7bd-8c6b-4b4c-866e-077a862c9f58.jpg?v=1782237048","url":"https:\/\/www.ebiohippo.com\/products\/pafah1b2-antibody-platelet-activating-factor-acetylhydrolase-ib-subunit-beta-bha17135688","provider":"BioHippo","version":"1.0","type":"link"}