{"product_id":"pag1-antibody-cbp-phosphoprotein-associated-with-glycosphingolipid-enriched-microdomains-1-bha17135969","title":"PAG1 Antibody \/ Cbp \/ Phosphoprotein associated with glycosphingolipid-enriched microdomains 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePAG1 Antibody \/ Cbp \/ Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 is a anti-PAG1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, plasma membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PAG1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePAG1 antibody detects Phosphoprotein associated with glycosphingolipid-enriched microdomains 1, also called Transmembrane phosphoprotein Cbp, a transmembrane adaptor protein that regulates Src family kinase signaling and immune receptor function. The UniProt recommended name is Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 (PAG1). This scaffold protein, also known as Csk-binding protein (CBP), localizes to lipid rafts in the plasma membrane and mediates inhibitory signaling by recruiting the tyrosine kinase CSK to suppress Src family kinases such as LCK and FYN.\u003cbr\u003e\u003cbr\u003eFunctionally, PAG1 antibody identifies a 432-amino-acid type I transmembrane protein containing multiple tyrosine residues that undergo phosphorylation following receptor activation. Phosphorylated PAG1 binds CSK, leading to phosphorylation and inactivation of Src kinases at their C-terminal regulatory tyrosine. This mechanism provides a critical negative feedback loop that fine-tunes T cell receptor (TCR) and B cell receptor (BCR) signaling, maintaining immune homeostasis.\u003cbr\u003e\u003cbr\u003eThe PAG1 gene is located on chromosome 8q21.13 and is expressed in hematopoietic cells, neurons, and various epithelial tissues. In T lymphocytes, PAG1 is enriched in membrane microdomains and associates with LAT, LCK, and CD45, modulating activation thresholds and signaling kinetics. Beyond the immune system, PAG1 participates in neuronal development, cell adhesion, and stress response signaling pathways.\u003cbr\u003e\u003cbr\u003ePathologically, dysregulated PAG1 expression contributes to altered immune signaling and oncogenic kinase activation. Downregulation of PAG1 has been observed in certain lymphomas and leukemias, allowing unchecked Src kinase activity. Conversely, overexpression may suppress cell motility and proliferation. Research using PAG1 antibody supports investigations into lipid raft-associated signaling, immune synapse organization, and signal attenuation mechanisms.\u003cbr\u003e\u003cbr\u003ePAG1 antibody is suitable for use in western blotting, immunoprecipitation, and immunofluorescence to detect native or phosphorylated PAG1 in cell lysates or tissue samples. It supports studies focused on immune receptor regulation, kinase signaling networks, and cancer biology. \u003cbr\u003e\u003cbr\u003eStructurally, PAG1 contains an extracellular immunoglobulin-like domain, a single transmembrane segment, and a cytoplasmic tail rich in tyrosine motifs. Its lipid raft localization depends on palmitoylation and interaction with glycosphingolipids. This antibody aids in elucidating PAG1's scaffolding functions that link plasma membrane signaling to intracellular kinase regulation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PAG1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PAG1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PAG1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PAG1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PAG1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047311008109,"sku":"FY13067","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c7ba74e4-3ff1-443e-8378-cf5f978c6103.jpg?v=1782237110","url":"https:\/\/www.ebiohippo.com\/products\/pag1-antibody-cbp-phosphoprotein-associated-with-glycosphingolipid-enriched-microdomains-1-bha17135969","provider":"BioHippo","version":"1.0","type":"link"}