{"product_id":"pam-antibody-fitc-conjugated-bha10532308","title":"PAM Antibody, FITC conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis polyclonal anti-PAM antibody raised in Rabbit, conjugated with FITC. Designed to detect PAM in Human for neuroscience research.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Recombinant Human Peptidyl-glycine alpha-amidating monooxygenase protein (338-497AA) — determines epitope; confirm sample prep compatibility.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary matching isotype IgG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal:\u003c\/strong\u003e Multiple epitopes = robust signal; lot-to-lot variability — include controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Match secondary, Protein A\/G, and isotype controls to IgG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Protein G purification):\u003c\/strong\u003e Enriches Ig classes; reduces non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFITC:\u003c\/strong\u003e Direct fluorescence (Ex490\/Em525); protect from light; use antifade.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003ePAM\u003c\/strong\u003e (also: AMD_HUMAN, PAL, PAM, Pancreatic peptidylglycine alpha amidating monooxygenase, Peptidyl alpha amidating enzyme, Peptidyl alpha hydroxyglycine alpha amidating lyase, Peptidyl-alpha-hydroxyglycine alpha-amidating lyase, Peptidylamidoglycolate lyase, Peptidylglycine 2 hydroxylase, Peptidylglycine alpha amidating monooxygenase, Peptidylglycine alpha hydroxylating monooxygenase, PHM) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current PAM annotation relevant to neuroscience.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003eRefer to product documentation for confirmed application data.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e IgG from Rabbit at equivalent concentration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Polyclonal: validate with KO\/KD controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Verify dilution linearity in your sample matrix.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Confirmed for Human; extrapolation requires validation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\u003c!-- Sources: UniProt, NCBI Gene, Cusabio, Human Protein Atlas, PubMed --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 ug","offer_id":53241132482925,"sku":"CSB-PA017417LC01HU-50UG","price":170.98,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53241224069485,"sku":"CSB-PA017417LC01HU-100UG","price":307.97,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Cusabio_Antibody_Placeholder_712e7f35-a6b8-4228-bcdf-1c38e46b94f4.jpg?v=1784927085","url":"https:\/\/www.ebiohippo.com\/products\/pam-antibody-fitc-conjugated-bha10532308","provider":"BioHippo","version":"1.0","type":"link"}