{"product_id":"pan-pkc-antibody-prkca-prkcb-prkcd-prkce-prkcg-prkcz-bha17135891","title":"Pan-PKC Antibody \/ PRKCA\/PRKCB\/PRKCD\/PRKCE\/PRKCG\/PRKCZ","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePan-PKC Antibody \/ PRKCA\/PRKCB\/PRKCD\/PRKCE\/PRKCG\/PRKCZ is a anti-Pan-PKC Rabbit antibody Recombinant Rabbit Monoclonal clone 32P28 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Pan-PKC\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32P28, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePan-PKC antibody detects multiple isoforms of Protein kinase C, including PRKCA, PRKCB, PRKCD, PRKCE, PRKCG, and PRKCZ. Protein kinase C represents a family of serine threonine kinases that play central roles in transducing signals from membrane receptors to diverse downstream pathways. These kinases regulate processes such as cell growth, differentiation, apoptosis, immune responses, and neurotransmission. Pan-PKC antibody provides a broad detection tool for studying Protein kinase C activity across its isoforms, enabling researchers to assess general pathway activation in cellular models and tissues.\u003cbr\u003e\u003cbr\u003eThe Protein kinase C family is divided into conventional, novel, and atypical classes based on their structural domains and activation requirements. Conventional isoforms such as PRKCA, PRKCB, and PRKCG require calcium and diacylglycerol for activation, while novel isoforms including PRKCD and PRKCE are calcium independent but still activated by diacylglycerol. Atypical isoforms such as PRKCZ do not respond to either calcium or diacylglycerol but are regulated by other lipid derived second messengers. Research using Pan-PKC antibody has shown that this kinase family integrates multiple extracellular signals, allowing cells to adapt rapidly to changing environments.\u003cbr\u003e\u003cbr\u003eAberrant Protein kinase C signaling is implicated in cancer, diabetes, cardiovascular disease, and neurological disorders. Overexpression or mutation of individual isoforms alters cell cycle progression, apoptosis thresholds, and stress responses. For example, PRKCA is frequently upregulated in breast cancer, while PRKCD contributes to apoptosis in lymphoid cells. Using Pan-PKC antibody allows researchers to detect total family expression or activation states, providing a comprehensive readout when isoform specificity is not required. This broad recognition is especially valuable in screening studies where pathway activity is assessed across multiple tissues and disease contexts.\u003cbr\u003e\u003cbr\u003ePan-PKC antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting enables detection of multiple isoforms in complex lysates, while immunohistochemistry highlights expression patterns across tissues such as brain, liver, and immune organs. Immunofluorescence demonstrates localization of Protein kinase C to membranes, cytoplasm, or nucleus depending on isoform activation state. Functional studies with Pan-PKC antibody reveal dynamic translocation patterns following receptor stimulation, underscoring the central role of this kinase family in signaling.\u003cbr\u003e\u003cbr\u003eBy supplying validated Pan-PKC antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=Pan-PKC - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Pan-PKC - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Pan-PKC - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/Pan-PKC - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Pan-PKC+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047308157293,"sku":"FY12989","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_18b65440-b31b-4bbc-b1d2-083bc5829122.jpg?v=1772019374","url":"https:\/\/www.ebiohippo.com\/products\/pan-pkc-antibody-prkca-prkcb-prkcd-prkce-prkcg-prkcz-bha17135891","provider":"BioHippo","version":"1.0","type":"link"}