{"product_id":"pan-pkc-antibody-prkca-prkcb-prkcz-bha17135737","title":"Pan-PKC Antibody \/ PRKCA\/PRKCB\/PRKCZ","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePan-PKC Antibody \/ PRKCA\/PRKCB\/PRKCZ is a anti-Pan-PKC Rabbit antibody Recombinant Rabbit Monoclonal clone 32P76 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Pan-PKC\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32P76, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, IP, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePan-PKC antibody detects protein kinase C isoforms alpha, beta, and zeta, encoded by the PRKCA, PRKCB, and PRKCZ genes. PKC proteins are a family of serine\/threonine kinases activated by diacylglycerol, calcium, and phospholipids. They regulate diverse processes including proliferation, apoptosis, migration, and secretion. The PKC family is divided into conventional, novel, and atypical subgroups based on activation requirements. PKC alpha belongs to the conventional subgroup requiring both calcium and diacylglycerol, PKC beta has two splice variants beta I and beta II, while PKC zeta is atypical and functions independently of calcium and diacylglycerol. By detecting multiple isoforms simultaneously, Pan-PKC antibody provides a broad tool for studying PKC biology.\u003cbr\u003e\u003cbr\u003ePan-PKC antibody is widely applied in cancer biology, immunology, and neuroscience. PKC alpha regulates proliferation and apoptosis in many cell types, PKC beta is critical for B cell receptor signaling, and PKC zeta participates in NF-kappaB signaling and polarity regulation. Together, these isoforms integrate signaling from growth factors, immune receptors, and stress pathways. By applying Pan-PKC antibody, researchers can examine global PKC expression and activity across diverse systems.\u003cbr\u003e\u003cbr\u003eApplications for Pan-PKC antibody include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blot assays detect multiple PKC isoforms in cell and tissue lysates, immunohistochemistry maps distribution in tumors and immune organs, and immunofluorescence reveals subcellular localization at membranes, cytoskeleton, and nuclei. These approaches allow characterization of PKC biology at multiple levels.\u003cbr\u003e\u003cbr\u003eDysregulation of PKC isoforms contributes to cancer, autoimmunity, diabetes, and cardiovascular disease. Overactivation of PKC promotes tumor growth, invasion, and angiogenesis, while loss of function impairs immune signaling and metabolic regulation. By detecting PKC isoforms broadly, Pan-PKC antibody supports research into how kinase networks contribute to disease. In leukemia, aberrant PKC activity modulates drug sensitivity, while in diabetes, PKC activation contributes to vascular complications.\u003cbr\u003e\u003cbr\u003ePKC isoforms also play roles in neuronal signaling, where they regulate neurotransmitter release and plasticity. By applying Pan-PKC antibody, scientists can study PKC activity in synaptic function and memory. Therapeutically, PKC inhibitors are under investigation for oncology and metabolic disorders. Monitoring PKC expression with antibody-based assays provides biomarkers for drug development and clinical research.\u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=Pan-PKC - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Pan-PKC - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Pan-PKC - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/Pan-PKC - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Pan-PKC+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047302783341,"sku":"FY12835","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_8dcbe7e5-38f1-4123-8728-36c274dcd800.jpg?v=1772019351","url":"https:\/\/www.ebiohippo.com\/products\/pan-pkc-antibody-prkca-prkcb-prkcz-bha17135737","provider":"BioHippo","version":"1.0","type":"link"}