{"product_id":"pan3-antibody-pan-deadenylation-complex-subunit-3-bha17135400","title":"PAN3 Antibody \/ PAN deadenylation complex subunit 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePAN3 Antibody \/ PAN deadenylation complex subunit 3 is a anti-PAN3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PAN3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePAN3 antibody detects PAB-dependent poly(A)-specific ribonuclease subunit 3, a component of the PAN deadenylation complex responsible for the removal of poly(A) tails from mRNA molecules. PAN3 forms a heterodimer with PAN2 to mediate mRNA turnover, regulating transcript stability and translation. The PAN3 antibody is widely used in studies of RNA metabolism, post-transcriptional control, and gene expression regulation.\u003cbr\u003e\u003cbr\u003ePAN3 is encoded by the PAN3 gene located on human chromosome 13q12.13. The protein is approximately 89 kilodaltons and characterized by coiled-coil, zinc finger, and RNA-binding motifs that support its role in mRNA degradation. Within the PAN2-PAN3 complex, PAN3 acts as a scaffold protein that recruits PAN2 to poly(A)-bound PABP (poly(A)-binding protein) and stimulates deadenylase activity. This process represents the first step in mRNA decay, preceding decapping and exonucleolytic degradation.\u003cbr\u003e\u003cbr\u003eThe PAN3 antibody detects an ~96 kilodalton protein by western blot and shows cytoplasmic punctate localization consistent with processing bodies (P-bodies) and stress granules. Functional assays reveal that PAN3, together with PAN2, regulates transcript half-life during stress, differentiation, and cell cycle transitions. Depletion of PAN3 stabilizes mRNAs and alters global translation, underscoring its central role in RNA homeostasis.\u003cbr\u003e\u003cbr\u003eBeyond mRNA decay, PAN3 contributes to early embryonic development and stress responses by coordinating the balance between translation and degradation. It interacts with the CCR4-NOT complex and RNA helicases, integrating deadenylation with other post-transcriptional mechanisms. \u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PAN3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PAN3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PAN3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PAN3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PAN3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047290134893,"sku":"FY12497","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_20ce2687-d442-477d-b261-f4c641dbca6f.jpg?v=1782237005","url":"https:\/\/www.ebiohippo.com\/products\/pan3-antibody-pan-deadenylation-complex-subunit-3-bha17135400","provider":"BioHippo","version":"1.0","type":"link"}