{"product_id":"parp-1-knockout-immortalized-mouse-heart-endothelial-hyko6-cell-line-bhc10901402","title":"PARP-1 Knockout Immortalized Mouse Heart Endothelial (HYKO6) Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003ePoly(ADP-ribose) polymerase 1 (PARP-1) is an ADP-ribosylating enzyme that plays a critical role in DNA damage repair, in addition to functioning as a context- specific regulator of transcription factors. Various pathophysiological conditions, such as reperfusion injury, endotoxic shock, diabetes and aging are associated with endothelial cell dysfunction mediated by PARP-1.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e PARP-1 Knockout Immortalized Mouse Heart Endothelial (HYKO6) Cell Line is supplied as an engineered cell line derived from Mouse heart.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, cobblestone\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). RPMI 1640 Medium (TM503) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in vascular biology, signaling, and assay development studies.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThe PARP-1-\/-Knockout Immortalized Mouse Heart Endothelial (HYKO6) Cell Line is derived from stable transformation of SV40 into primary 9-10 week old 129\/Sv x C57BL\/6 mouse heart endothelial cells deficient for PARP-1. This cell line is capable of morphologic differentiation in capillary-like structures (CLS), similar to its primary counterparts, as shown by branching and anastomosing cords of cell networks and tube formation after 12 hours culturing on Matrigel. Moreover, HYKO6 responds to cytokine stimulation and has been shown to exhibit increased VCAM-1 and E-selectin expression after TNFα stimulation, making this cell line an important tool in the elucidation of PARP-1 signaling pathway involved in endothelial cell dysfunction and molecular mechanisms controlling cell gene expression in response to inflammatory stimuli.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEngineered cell lines are widely used for reporter-based readouts, perturbation studies, and assay optimization in reproducible culture systems.\u003c\/li\u003e\n\u003cli\u003eReporter or transgene-bearing models are often compared with matched parental or control cells to interpret signal changes in context.\u003c\/li\u003e\n\u003cli\u003eExpression trends are typically evaluated alongside passage number, selection pressure, and baseline growth behavior.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). RPMI 1640 Medium (TM503) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePopulation Doubling Time (h):\u003c\/strong\u003e 30 - 40\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 20,000 - 30,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180525511021,"sku":"T3031","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/L4u8BH5AcU4aJhs5ersmf0Zkhy3JAJPeQFCtiW78.png?v=1774957818","url":"https:\/\/www.ebiohippo.com\/products\/parp-1-knockout-immortalized-mouse-heart-endothelial-hyko6-cell-line-bhc10901402","provider":"BioHippo","version":"1.0","type":"link"}