{"product_id":"parp1-antibody-poly-adp-ribose-polymerase-1-bha17135914","title":"PARP1 Antibody \/ Poly ADP-ribose polymerase 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePARP1 Antibody \/ Poly ADP-ribose polymerase 1 is a anti-PARP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PARP1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePARP1 antibody detects Poly [ADP-ribose] polymerase 1, a nuclear enzyme essential for DNA repair, chromatin remodeling, and transcriptional regulation. The UniProt recommended name is Poly [ADP-ribose] polymerase 1 (PARP1). This enzyme catalyzes the poly-ADP-ribosylation of nuclear proteins in response to DNA strand breaks, recruiting DNA repair machinery and maintaining genomic integrity. PARP1 plays a dual role as both a DNA damage sensor and a regulator of chromatin structure.\u003cbr\u003e\u003cbr\u003eFunctionally, PARP1 antibody identifies a 1014-amino-acid nuclear protein containing three zinc-finger DNA-binding domains, a BRCT motif, and a catalytic PARP domain. Upon binding to damaged DNA, PARP1 catalyzes the transfer of ADP-ribose units from NAD+ to target proteins, forming long poly-ADP-ribose (PAR) chains. These modifications facilitate the recruitment of DNA repair factors such as XRCC1, DNA ligase III, and DNA polymerase beta. PARP1 activity is a key step in the base excision repair (BER) pathway, ensuring timely resolution of single-strand breaks.\u003cbr\u003e\u003cbr\u003eThe PARP1 gene is located on chromosome 1q42.12 and is ubiquitously expressed in proliferative and differentiated tissues. It modulates chromatin relaxation by poly-ADP-ribosylating histones, thereby granting access to repair and transcriptional complexes. Beyond DNA repair, PARP1 regulates transcription by interacting with nuclear receptors and chromatin modifiers, influencing inflammation, cell cycle, and apoptosis. PARP1 activity is tightly regulated by DNA damage signals and cellular NAD+ levels.\u003cbr\u003e\u003cbr\u003eIn apoptosis, excessive PARP1 activation during oxidative or genotoxic stress leads to NAD+ and ATP depletion, contributing to programmed necrosis (parthanatos). PARP inhibitors, such as olaparib and niraparib, target PARP1 enzymatic activity to enhance the cytotoxicity of DNA-damaging agents, forming the basis for synthetic lethality in BRCA-deficient cancers. Overactivation or dysregulation of PARP1 has been linked to neurodegeneration, ischemic injury, and cancer progression.\u003cbr\u003e\u003cbr\u003ePARP1 antibody is widely used in molecular biology, oncology, and DNA repair research. It is suitable for western blotting, immunohistochemistry, and chromatin immunoprecipitation to detect endogenous or modified PARP1. This antibody supports studies of DNA repair, PARP inhibitor mechanisms, and nuclear signaling pathways. In cancer models, PARP1 detection is crucial for assessing DNA damage response and drug sensitivity.\u003cbr\u003e\u003cbr\u003eStructurally, PARP1 contains DNA-binding zinc fingers, a WGR domain involved in DNA interaction, and a catalytic domain responsible for NAD+ hydrolysis and ADP-ribose polymerization. Its activity is regulated through automodification, phosphorylation, and proteolytic cleavage during apoptosis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PARP1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PARP1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PARP1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PARP1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PARP1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047310156141,"sku":"FY13012","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_acc55b61-3692-4181-ab6c-af8c00d55c8a.jpg?v=1782237066","url":"https:\/\/www.ebiohippo.com\/products\/parp1-antibody-poly-adp-ribose-polymerase-1-bha17135914","provider":"BioHippo","version":"1.0","type":"link"}