{"product_id":"parp11-antibody-poly-adp-ribose-polymerase-11-bha17135415","title":"PARP11 Antibody \/ Poly ADP-ribose polymerase 11","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePARP11 Antibody \/ Poly ADP-ribose polymerase 11 is a anti-PARP11 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PARP11\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ICC\/IF, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePARP11 antibody detects Poly(ADP-ribose) polymerase 11, a mono-ADP-ribosyltransferase enzyme that modifies proteins through ADP-ribosylation, influencing their stability, localization, and activity. PARP11 belongs to the PARP family but, unlike canonical poly(ADP-ribose) polymerases, primarily catalyzes mono-ADP-ribose addition. This post-translational modification regulates processes such as nuclear transport, innate immunity, and antiviral defense. The PARP11 antibody is used to study protein modification signaling, interferon responses, and nuclear envelope dynamics.\u003cbr\u003e\u003cbr\u003ePARP11 is encoded by the PARP11 gene on human chromosome 12q13.11. The protein is approximately 39 kilodaltons and characterized by an N-terminal WWE domain, which mediates protein-protein interactions, and a C-terminal catalytic domain containing the conserved histidine-tyrosine-glutamate motif typical of ADP-ribosyltransferases. PARP11 localizes predominantly to the nuclear envelope and cytoplasmic membrane-associated compartments.\u003cbr\u003e\u003cbr\u003eThe PARP11 antibody detects a 39 kilodalton band by western blot and shows nuclear rim and cytoplasmic punctate staining patterns in immunofluorescence microscopy. PARP11 functions in antiviral defense by ADP-ribosylating host and viral proteins involved in interferon signaling, modulating downstream immune activation. It targets nuclear pore complex components and transport receptors, thereby altering nucleocytoplasmic trafficking during stress or infection. Studies have demonstrated that PARP11 negatively regulates type I interferon responses by promoting degradation of TBK1 and IFNAR1, helping fine-tune innate immune activity.\u003cbr\u003e\u003cbr\u003ePARP11 also influences ubiquitin-proteasome and SUMOylation pathways through crosstalk with other post-translational modifiers. It plays a role in maintaining nuclear envelope structure and in mitotic progression. Overexpression of PARP11 has been associated with enhanced cell survival under stress, whereas its inhibition sensitizes cells to DNA damage and viral infection. Structural studies suggest that PARP11 recognizes unique NAD+ analogs distinct from other PARP family members, indicating potential for selective drug targeting.\u003cbr\u003e\u003cbr\u003eEmerging evidence links PARP11 to cancer, viral replication, and neurodegenerative processes. In hepatocellular and colorectal carcinomas, PARP11 overexpression correlates with immune evasion phenotypes and resistance to interferon-based therapies. In neurons, altered ADP-ribosylation by PARP11 may affect axonal transport or synaptic signaling.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PARP11 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PARP11 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PARP11 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PARP11 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PARP11+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047291642221,"sku":"FY12512","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_55771fae-70b1-4c14-9ee3-835a93cd27eb.jpg?v=1782237019","url":"https:\/\/www.ebiohippo.com\/products\/parp11-antibody-poly-adp-ribose-polymerase-11-bha17135415","provider":"BioHippo","version":"1.0","type":"link"}