{"product_id":"parp15-antibody-poly-adp-ribose-polymerase-15-bha17135350","title":"PARP15 Antibody \/ Poly ADP-ribose polymerase 15","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePARP15 Antibody \/ Poly ADP-ribose polymerase 15 is a anti-PARP15 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PARP15\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePARP15 antibody recognizes Poly (ADP-ribose) polymerase 15, an enzyme belonging to the PARP family involved in ADP-ribosylation, a post-translational modification regulating transcription, DNA repair, and stress responses. Unlike the classical DNA repair PARPs, PARP15 functions primarily in transcriptional repression and RNA processing. It possesses both mono-ADP-ribosyltransferase and macro domains, allowing it to modify specific target proteins. The PARP15 antibody is an essential reagent for studies of ADP-ribosylation signaling, chromatin dynamics, and cellular stress adaptation.\u003cbr\u003e\u003cbr\u003ePARP15 is encoded by the PARP15 gene located on human chromosome 3q21.1. The protein comprises two N-terminal macro domains, a WWE domain involved in protein�protein interactions, and a C-terminal catalytic PARP domain. PARP15 localizes mainly to the nucleus and cytoplasmic granules, where it may regulate mRNA metabolism and response to stress. It has been implicated in cell survival pathways, as well as in the regulation of transcription factors under conditions of nutrient deprivation or oxidative stress.\u003cbr\u003e\u003cbr\u003eResearch using the PARP15 antibody has demonstrated its involvement in cancer cell proliferation and inflammation. Aberrant expression of PARP15 has been observed in lymphomas and certain solid tumors, where it may contribute to altered transcriptional control and resistance to apoptosis. Western blot analysis typically detects a band at approximately 74�78 kDa. Immunocytochemical staining shows both nuclear and cytoplasmic localization, depending on cell type and stress conditions. Functional studies suggest that PARP15 can auto-modify and regulate its own enzymatic activity, similar to other PARP family members.\u003cbr\u003e\u003cbr\u003eIn recent years, PARP15 has attracted attention as part of the broader ADP-ribosylation signaling network, which includes enzymes, hydrolases, and binding proteins that control protein modification during stress responses. The antibody supports exploration of this enzyme's role in RNA granule formation, viral defense, and oncogenic signaling pathways.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PARP15 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PARP15 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PARP15 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PARP15 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PARP15+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047289577837,"sku":"FY12447","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_24dad26a-b096-40e5-9dca-c220393da6ab.jpg?v=1782236999","url":"https:\/\/www.ebiohippo.com\/products\/parp15-antibody-poly-adp-ribose-polymerase-15-bha17135350","provider":"BioHippo","version":"1.0","type":"link"}