{"product_id":"parp16-antibody-poly-adp-ribose-polymerase-16-bha17135396","title":"PARP16 Antibody \/ Poly ADP-ribose polymerase 16","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePARP16 Antibody \/ Poly ADP-ribose polymerase 16 is a anti-PARP16 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm (ER).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PARP16\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePARP16 antibody detects Poly(ADP-ribose) polymerase 16, a membrane-associated mono-ADP-ribosyltransferase that regulates the unfolded protein response (UPR) and endoplasmic reticulum (ER) stress signaling. PARP16 belongs to the PARP family of enzymes involved in ADP-ribosylation, a post-translational modification that controls protein stability and signaling. The PARP16 antibody is commonly used in studies of ER stress, apoptosis, and ADP-ribosylation-dependent signaling pathways.\u003cbr\u003e\u003cbr\u003ePARP16 is encoded by the PARP16 gene located on human chromosome 15q21.2. The protein is approximately 38 kilodaltons and uniquely localized to the ER membrane, where it functions as a tail-anchored enzyme with its catalytic domain facing the cytoplasm. Unlike canonical PARPs that catalyze poly(ADP-ribosylation), PARP16 primarily transfers single ADP-ribose moieties to substrate proteins (mono-ADP-ribosylation). This modification modulates the activity of key ER stress sensors, including PERK and IRE1-alpha, enhancing their signaling output during the UPR.\u003cbr\u003e\u003cbr\u003eThe PARP16 antibody typically detects a 38 kilodalton protein by western blot, and immunofluorescence confirms its ER localization. Functional studies demonstrate that PARP16 activity is induced upon ER stress caused by misfolded protein accumulation or chemical perturbation. Activated PARP16 promotes phosphorylation and activation of PERK and IRE1-alpha, leading to downstream transcriptional responses that restore proteostasis. Inhibition or depletion of PARP16 compromises the UPR, resulting in increased sensitivity to ER stress and enhanced apoptosis.\u003cbr\u003e\u003cbr\u003eBeyond its role in ER stress regulation, PARP16 contributes to cancer cell survival and resistance to chemotherapeutic agents that induce proteotoxic stress. Overexpression has been reported in hepatocellular carcinoma, breast cancer, and melanoma, where it supports adaptive stress signaling. Conversely, loss of PARP16 sensitizes cells to stress-induced death, making it a potential therapeutic target.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PARP16 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PARP16 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PARP16 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PARP16 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PARP16+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047290429805,"sku":"FY12493","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_333b03c1-c3ac-4038-a888-a25e59c74e6d.jpg?v=1782237006","url":"https:\/\/www.ebiohippo.com\/products\/parp16-antibody-poly-adp-ribose-polymerase-16-bha17135396","provider":"BioHippo","version":"1.0","type":"link"}