{"product_id":"pask-antibody-pas-kinase-bha17135601","title":"PASK Antibody \/ PAS kinase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePASK Antibody \/ PAS kinase is a anti-PASK Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Flow cytometry (FACS) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PASK\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePASK antibody detects PAS domain-containing serine\/threonine-protein kinase, an evolutionarily conserved enzyme that functions as a nutrient and energy sensor linking cellular metabolism with transcriptional control. Encoded by the PASK gene on chromosome 2q37.3, this kinase contains a C-terminal catalytic domain and N-terminal PAS (Per-Arnt-Sim) domains that sense changes in oxygen, redox potential, and small-molecule metabolites. PASK acts as a molecular integrator that coordinates energy homeostasis, glucose metabolism, and mitochondrial biogenesis in response to nutrient availability. The PAS domains enable regulation of its kinase activity through allosteric mechanisms, allowing PASK to respond dynamically to metabolic states.\u003cbr\u003e\u003cbr\u003ePASK is broadly expressed in liver, skeletal muscle, pancreatic islets, and the brain, tissues that exhibit strong metabolic plasticity. In hepatocytes, PASK influences glycogen synthesis and gluconeogenic pathways, while in pancreatic beta cells it modulates insulin secretion. Mice lacking PASK exhibit altered glucose tolerance, reduced hepatic glycogen storage, and impaired mitochondrial function, demonstrating its role in metabolic adaptation. PASK phosphorylates transcriptional coactivators such as PGC-1alpha and CREB-regulated transcriptional coactivator 2 (CRTC2), linking nutrient signaling with gene expression that governs oxidative metabolism.\u003cbr\u003e\u003cbr\u003eThe PASK antibody is used in metabolic research to detect PASK expression and activation across tissues. Western blotting typically identifies a ~160 kilodalton band corresponding to the full-length kinase, while immunofluorescence localizes it to the cytoplasm and nucleus depending on cell type. In mammalian systems, PASK activity rises during fasting and decreases in nutrient-replete conditions, reflecting its regulatory role in energy balance. Because dysregulation of PASK signaling has been associated with diabetes, obesity, and mitochondrial dysfunction, this antibody provides an important tool for investigating metabolic signaling networks.\u003cbr\u003e\u003cbr\u003eRecent studies also implicate PASK in circadian rhythm regulation and differentiation of muscle and stem cells. Its PAS domains act as environmental sensors, adjusting transcriptional programs to oxygen and redox changes. PASK interacts with AMPK and mTOR pathways, highlighting its integration into major metabolic signaling circuits. The PASK antibody enables researchers to explore these regulatory interactions and to characterize PASK as a potential therapeutic target in metabolic disease and aging.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PASK - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PASK - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PASK - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PASK - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PASK+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047298031981,"sku":"FY12698","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_95833c0e-2318-423e-874d-03eab2a3dc04.jpg?v=1782237035","url":"https:\/\/www.ebiohippo.com\/products\/pask-antibody-pas-kinase-bha17135601","provider":"BioHippo","version":"1.0","type":"link"}