{"product_id":"pax3-antibody-paired-box-protein-pax-3-bha17136138","title":"PAX3 Antibody \/ Paired box protein Pax-3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePAX3 Antibody \/ Paired box protein Pax-3 is a anti-PAX3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PAX3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePAX3 antibody detects Paired box protein Pax-3, a transcription factor essential for embryonic development, neural crest differentiation, and myogenesis. The UniProt recommended name is Paired box protein Pax-3 (PAX3). This nuclear DNA-binding protein functions as a master regulator of gene expression programs controlling cell fate and migration during early development.\u003cbr\u003e\u003cbr\u003eFunctionally, PAX3 antibody identifies a 479-amino-acid transcription factor containing both a paired DNA-binding domain and a homeobox domain. PAX3 regulates genes involved in neural crest formation, muscle lineage specification, and melanocyte development. It acts in concert with cofactors such as SOX10, MITF, and FOXO1 to coordinate developmental gene networks. In muscle precursors, PAX3 activates MyoD and MYF5, driving myogenic commitment and differentiation.\u003cbr\u003e\u003cbr\u003eThe PAX3 gene is located on chromosome 2q36.1 and is highly expressed in the dorsal neural tube, somites, and limb buds during embryogenesis. It remains active in satellite cells and neural crest-derived lineages in adult tissues. PAX3 activity is modulated by phosphorylation, sumoylation, and interaction with chromatin-remodeling complexes.\u003cbr\u003e\u003cbr\u003ePathologically, mutations in PAX3 cause Waardenburg syndrome types 1 and 3, characterized by hearing loss and pigmentary abnormalities. Chromosomal translocations involving PAX3 (such as PAX3-FOXO1 fusions) drive alveolar rhabdomyosarcoma, a pediatric soft tissue cancer. Aberrant PAX3 signaling also contributes to melanoma progression by enhancing cell migration and survival. Research using PAX3 antibody supports studies in developmental biology, stem cell regulation, and cancer genetics.\u003cbr\u003e\u003cbr\u003ePAX3 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect transcription factors in neural and muscle tissues. \u003cbr\u003e\u003cbr\u003eStructurally, Paired box protein Pax-3 contains two DNA-binding domains-a paired domain and a homeodomain-linked by a flexible region that allows diverse DNA sequence recognition. The C-terminal transactivation domain mediates recruitment of coactivators and repressors. This antibody enables precise analysis of PAX3's regulatory functions in development and disease.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PAX3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PAX3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PAX3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PAX3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PAX3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047318610285,"sku":"FY13236","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_97ff7eb3-bf66-4398-acdd-0cb5432bbe4b.jpg?v=1782237078","url":"https:\/\/www.ebiohippo.com\/products\/pax3-antibody-paired-box-protein-pax-3-bha17136138","provider":"BioHippo","version":"1.0","type":"link"}