{"product_id":"pax6-reporter-lentivirus-bhv19400220","title":"PAX6 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ePAX6 is a paired-box transcription factor that serves as a master regulator of eye development and is also essential for the formation of the central nervous system, olfactory system, and pancreas. PAX6 governs cell fate specification and differentiation, often acting combinatorially with partner factors such as SOX2 and SOX3 at tissue-specific enhancers. The conserved, surface ectoderm-specific LE9 enhancer contains a PAX6 response element through which PAX6 and SOX2\/SOX3 act synergistically to drive lens development. Because PAX6 dosage is tightly controlled, mutations cause developmental eye disorders such as aniridia, underscoring its central role in developmental biology and neuroscience.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe PAX6 Reporter Lentivirus is a transcription-factor reporter system for robust detection of human and mouse PAX6 activity. It is engineered to read out the synergistic activity of PAX6 with SOX2\/SOX3 using tandem repeats of the PAX6 response element from the conserved LE9 enhancer, coupled to a minimal TATA-box promoter and an upstream enhancer that maximizes signal-to-noise to drive a reporter gene (including BFP2, d2GFP, EGFP, GFP, mCherry, RFP, firefly, Gaussia, or Renilla luciferase). A constitutively expressed selection marker (hygromycin, zeocin, puromycin, or blasticidin) supports stable polyclonal cell line generation. Stable lentiviral integration provides consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved material, with readout by microscopy, flow cytometry, or luminometry. It is used to study PAX6 activity in developmental biology and neuroscience. Supplied as purified lentiviral particles.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP + Firefly Luc, mCherry, Renilla Luc, RFP + Firefly Luc, BFP2, Gaussia Luc, GFP, RFP reporter gene under the control of tandem consensus response elements specific for the PAX6 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Hygromycin, Zeocin, Puromycin, Blasticidin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251622863213,"sku":"LTV-0111-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53362029527405,"sku":"LTV-0111-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53362029560173,"sku":"LTV-0111-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53362029592941,"sku":"LTV-0111-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53362029625709,"sku":"LTV-0111-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53362029658477,"sku":"LTV-0111-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/PAX6-Reporter-Figure.jpg?v=1776934151","url":"https:\/\/www.ebiohippo.com\/products\/pax6-reporter-lentivirus-bhv19400220","provider":"BioHippo","version":"1.0","type":"link"}