{"product_id":"pax7-antibody-paired-box-protein-7-bha17135408","title":"PAX7 Antibody \/ Paired box protein 7","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePAX7 Antibody \/ Paired box protein 7 is a anti-PAX7 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PAX7\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePAX7 antibody detects Paired box protein 7, a transcription factor critical for myogenesis, neural crest formation, and satellite cell maintenance. PAX7 belongs to the paired box family of transcriptional regulators that control early developmental programs through DNA binding and transcriptional activation. The PAX7 antibody is widely used in developmental and muscle biology to study stem cell regulation and tissue regeneration.\u003cbr\u003e\u003cbr\u003ePAX7 is encoded by the PAX7 gene located on human chromosome 1p36.13. The protein contains a paired DNA-binding domain, a homeodomain, and a C-terminal transactivation domain. During embryonic development, PAX7 is expressed in neural crest cells and the myogenic progenitor population of the somites. In adults, it marks quiescent muscle satellite cells responsible for postnatal muscle repair and regeneration.\u003cbr\u003e\u003cbr\u003eThe PAX7 antibody identifies a 55 kilodalton nuclear protein by western blot and demonstrates strong nuclear staining in satellite cells and developing muscle fibers. Loss of PAX7 disrupts myogenic lineage specification, resulting in impaired muscle growth. Functionally, PAX7 activates myogenic regulatory factors such as MYOD and MYF5 and promotes self-renewal of satellite cells through repression of differentiation pathways.\u003cbr\u003e\u003cbr\u003eBeyond muscle tissue, PAX7 contributes to neurogenesis, pituitary development, and craniofacial patterning. Dysregulation of PAX7 is linked to rhabdomyosarcoma, where gene fusions with FOXO1 create oncogenic transcriptional drivers.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PAX7 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PAX7 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PAX7 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PAX7 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PAX7+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047291019629,"sku":"FY12505","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_29f82e9d-4475-4454-85f6-1278682597cb.jpg?v=1782237013","url":"https:\/\/www.ebiohippo.com\/products\/pax7-antibody-paired-box-protein-7-bha17135408","provider":"BioHippo","version":"1.0","type":"link"}