{"product_id":"pbcv-dna-ligase-25-u-mu-l-bhz20800111","title":"PBCV DNA Ligase (25 U\/µL)","description":"\u003cp class=\"p\"\u003e\u003cspan\u003ePBCV DNA Ligase, also known as SplintR ligase, PBCV-1 DNA Ligase, or Chlorella virus DNA ligase, is an ATP-dependent DNA ligase that efficiently catalyzes the ligation reaction of two adjacent DNA strands that are complementary to a single stranded RNA. In these two adjacent DNA strands, the one providing the 3' hydroxyl group is called the acceptor DNA strand, while the one providing the 5' phosphate group is called the donor DNA strand. During this ligation process, a complementary RNA strand acts as a \"splint\" or \"scaffold\" for the two DNA strands. The activity of PBCV DNA Ligase is much higher than that of traditional T4 DNA ligase and shows a strong affinity for DNA substrates with RNA as a splint (apparent Km = 1 nM, where a smaller Km value indicates a stronger affinity). It can detect unique RNA species at the subnanomolar level in complex mixtures, making it suitable for target enrichment processes in applications such as high-sensitivity RNA detection, next-generation sequencing, and molecular diagnostics.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eFeatures\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eNo residual nuclease, endonuclease, RNase, or phosphatase\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eApplications\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003eDetection of microRNAs and other small RNAs based on probe method\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003eConnecting single-stranded DNA through \"clamp\" fixation by complementary RNA sequences\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003eUsing DNA probes to achieve the detection of specific RNA\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003eDetection of SNP or Alternative Splicing\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003eRASL-seq analysis\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eSpecifications\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" class=\"MsoTableGrid\" style=\"width: 100.08%; height: 258.153px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 55.1944px;\"\u003e\n\u003ctd style=\"width: 37.1692%; height: 55.1944px;\" valign=\"top\" width=\"207\"\u003e\n\u003cp class=\"p\"\u003eSource\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 62.7907%; height: 55.1944px;\" valign=\"top\" width=\"434\"\u003e\n\u003cp class=\"MsoNormal\"\u003eThe DNA Ligase gene from PBCV-1 originating from Chlorella virus expressed in E.coli\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36.4861px;\"\u003e\n\u003ctd style=\"width: 37.1692%; height: 36.4861px;\" valign=\"top\" width=\"207\"\u003e\n\u003cp class=\"MsoNormal\"\u003eMolecular Weight\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 62.7907%; height: 36.4861px;\" valign=\"top\" width=\"434\"\u003e\n\u003cp class=\"MsoNormal\"\u003e34 KD\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36.4861px;\"\u003e\n\u003ctd style=\"width: 37.1692%; height: 36.4861px;\" valign=\"top\" width=\"207\"\u003e\n\u003cp class=\"MsoNormal\"\u003eConcentration\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 62.7907%; height: 36.4861px;\" valign=\"top\" width=\"434\"\u003e\n\u003cp class=\"MsoNormal\"\u003e25 U\/µL\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 94.3889px;\"\u003e\n\u003ctd style=\"width: 37.1692%; height: 94.3889px;\" valign=\"top\" width=\"207\"\u003e\n\u003cp class=\"MsoNormal\"\u003eUnit Definition\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 62.7907%; height: 94.3889px;\" valign=\"top\" width=\"434\"\u003e\n\u003cp class=\"MsoNormal\"\u003eOne unit is defined as the enzyme amount required to achieve at least 50% completion of the ligation of 2 pmol FAM-labeled DNA:RNA hybrid substrate within 15 min at 25℃ in 1×PBCV Ligase buffer\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5972px;\"\u003e\n\u003ctd style=\"width: 37.1692%; height: 35.5972px;\" valign=\"top\" width=\"207\"\u003e\n\u003cp class=\"MsoNormal\"\u003eInactivation Conditions\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 62.7907%; height: 35.5972px;\" valign=\"top\" width=\"434\"\u003e\n\u003cp class=\"MsoNormal\"\u003e65℃, 20 min\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eComponents\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"23.5000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003eComponent No.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"34.5400%\"\u003e\n\u003cp class=\"MsoNormal\"\u003eName\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"20.9800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e14962ES80\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"20.9800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e14962ES90\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"23.5000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e14962-A\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"34.5400%\"\u003e\n\u003cp class=\"MsoNormal\"\u003ePBCV DNA ligase (25 U\/µL)\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"20.9800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e50 µL\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"20.9800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e200 µL\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"23.5000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e14962-B\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"34.5400%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e10 x PBCV Ligase buffer\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"20.9800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e1 mL\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"20.9800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e1 mL\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eShipping and Storage\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\"\u003eThe products should be stored at -25℃ ~ -15℃ for 2 years.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"1250 U","offer_id":53294149796205,"sku":"14962ES80","price":155.0,"currency_code":"USD","in_stock":true},{"title":"5000 U","offer_id":53294155956589,"sku":"14962ES90","price":605.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/52939fc6a080c1414ba9f972209748bb.webp?v=1778112895","url":"https:\/\/www.ebiohippo.com\/products\/pbcv-dna-ligase-25-u-mu-l-bhz20800111","provider":"BioHippo","version":"1.0","type":"link"}