{"product_id":"pbpa-antibody-biotin-conjugated-bha10529863","title":"pbpA Antibody, Biotin conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis polyclonal anti-pbpA antibody raised in Rabbit, conjugated with Biotin, tested in ELISA. Designed to detect pbpA in Clostridium botulinum for others research.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Recombinant Clostridium botulinum Penicillin-binding protein 1A protein (663-830AA) — determines epitope; confirm sample prep compatibility.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary matching isotype IgG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal:\u003c\/strong\u003e Multiple epitopes = robust signal; lot-to-lot variability — include controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Match secondary, Protein A\/G, and isotype controls to IgG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Protein G purification):\u003c\/strong\u003e Enriches Ig classes; reduces non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiotin:\u003c\/strong\u003e Streptavidin\/avidin amplification; block endogenous biotin in biotin-rich samples.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003epbpA\u003c\/strong\u003e (also: pbpA, CBO3083, CLC_2985, Penicillin-binding protein 1A, PBP1a) [Includes: Penicillin-insensitive transglycosylase, EC 2.4.1.129, Peptidoglycan TGase), Penicillin-sensitive transpeptidase, EC 3.4.16.4, DD-transpeptidase)]) — studied in Clostridium botulinum systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current pbpA annotation relevant to others.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Quantify soluble target; ensure samples within the linear detection range.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e IgG from Rabbit at equivalent concentration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Polyclonal: validate with KO\/KD controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Verify dilution linearity in your sample matrix.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Confirmed for Clostridium botulinum; extrapolation requires validation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\u003c!-- Sources: UniProt, NCBI Gene, Cusabio, Human Protein Atlas, PubMed --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 ug","offer_id":53240875221357,"sku":"CSB-PA401992HD01CWV-50UG","price":170.98,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53240978276717,"sku":"CSB-PA401992HD01CWV-100UG","price":307.97,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Cusabio_Antibody_Placeholder_9aedb8e4-d94a-40b1-9224-58b66465c376.jpg?v=1784926967","url":"https:\/\/www.ebiohippo.com\/products\/pbpa-antibody-biotin-conjugated-bha10529863","provider":"BioHippo","version":"1.0","type":"link"}