{"product_id":"pbrm1-antibody-polybromo-1-bha17136092","title":"PBRM1 Antibody \/ Polybromo 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePBRM1 Antibody \/ Polybromo 1 is a anti-PBRM1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PBRM1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePBRM1 antibody detects Polybromo-1, a chromatin-remodeling factor and tumor suppressor that regulates gene expression through nucleosome repositioning. The UniProt recommended name is Polybromo-1 (PBRM1). This large nuclear protein functions as a subunit of the PBAF (polybromo-associated BRG1-associated factor) subtype of the SWI\/SNF chromatin-remodeling complex, which modulates chromatin accessibility to control transcription, differentiation, and DNA repair.\u003cbr\u003e\u003cbr\u003eFunctionally, PBRM1 antibody identifies a 1,709-amino-acid protein containing six bromodomains, two BAH domains, and one HMG-box domain that recognize acetylated lysine residues on histones. These interactions recruit the PBAF complex to specific promoters, enabling ATP-dependent chromatin remodeling and transcriptional regulation. PBRM1 supports cellular homeostasis by maintaining chromatin architecture and transcriptional fidelity.\u003cbr\u003e\u003cbr\u003eThe PBRM1 gene is located on chromosome 3p21.1 and is highly expressed in kidney, liver, and lung tissues. It acts as a critical tumor suppressor by opposing oncogenic transcriptional programs, particularly in renal and hepatic cells.\u003cbr\u003e\u003cbr\u003ePathologically, PBRM1 mutations are frequently observed in clear cell renal cell carcinoma (ccRCC), intrahepatic cholangiocarcinoma, and other malignancies. Loss of PBRM1 disrupts chromatin remodeling, leading to transcriptional dysregulation and uncontrolled cell proliferation. Research using PBRM1 antibody supports studies in epigenetics, tumor suppression, and chromatin dynamics.\u003cbr\u003e\u003cbr\u003ePBRM1 antibody is validated for western blotting, immunoprecipitation, and immunohistochemistry to detect chromatin-remodeling complex components. \u003cbr\u003e\u003cbr\u003eStructurally, Polybromo-1 features multiple bromodomains that bind acetylated histones and a modular organization that promotes assembly with PBAF complex members such as BRD7 and SMARCA4. This antibody facilitates exploration of PBRM1's role in chromatin organization and gene silencing.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PBRM1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PBRM1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PBRM1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PBRM1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PBRM1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047314645357,"sku":"FY13190","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_a3ab3871-d55e-4605-baae-cc862c3b3cd8.jpg?v=1782237110","url":"https:\/\/www.ebiohippo.com\/products\/pbrm1-antibody-polybromo-1-bha17136092","provider":"BioHippo","version":"1.0","type":"link"}