{"product_id":"pbx4-antibody-pre-b-cell-leukemia-transcription-factor-4-bha17135269","title":"PBX4 Antibody \/ Pre-B-cell leukemia transcription factor 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePBX4 Antibody \/ Pre-B-cell leukemia transcription factor 4 is a anti-PBX4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PBX4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe PBX4 antibody targets Pre-B-cell leukemia transcription factor 4, a nuclear homeobox protein encoded by the PBX4 gene. PBX4 is a member of the TALE (three amino acid loop extension) class of homeodomain transcription factors that regulate gene expression during development, differentiation, and organogenesis. By forming heterodimeric or trimeric complexes with HOX and MEIS family proteins, Pre-B-cell leukemia transcription factor 4 modulates the transcription of genes involved in skeletal formation, hematopoiesis, and reproductive system development. The PBX4 antibody provides a powerful reagent for exploring transcriptional control mechanisms mediated by this developmental regulator.\u003cbr\u003e\u003cbr\u003ePre-B-cell leukemia transcription factor 4 contains a conserved homeodomain that binds DNA at specific sequence motifs and an N-terminal domain that mediates protein-protein interactions. It functions both as a transcriptional activator and repressor depending on its cofactors and target promoters. The PBX4 antibody enables visualization of nuclear expression and quantification of PBX4 across tissue types, supporting studies into gene regulatory networks controlling pattern formation and organ differentiation.\u003cbr\u003e\u003cbr\u003ePBX family proteins act as cofactors for HOX transcription factors, enhancing DNA-binding specificity and transcriptional activation of developmental genes. PBX4 is expressed in gonadal, skeletal, and neural tissues, where it helps coordinate morphogenesis and cell lineage specification. The PBX4 antibody supports detailed characterization of these expression domains in embryonic and adult tissues. Functional studies suggest PBX4 may also influence germ cell maturation and fertility by regulating genes involved in testis differentiation.\u003cbr\u003e\u003cbr\u003eIn cancer biology, aberrant PBX expression contributes to oncogenesis by altering transcriptional programs that control proliferation and differentiation. Elevated PBX4 levels have been linked to tumor growth, epithelial-mesenchymal transition, and metastasis in several cancers. The PBX4 antibody enables detection of these expression changes and supports functional studies investigating its role in oncogenic transcriptional regulation. Western blotting and immunohistochemical analysis using this antibody reveal nuclear localization typical of transcription factors.\u003cbr\u003e\u003cbr\u003eThe PBX4 antibody performs effectively in immunofluorescence, immunohistochemistry, and chromatin immunoprecipitation, providing versatility across molecular and developmental biology applications.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PBX4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PBX4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PBX4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PBX4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PBX4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047287677293,"sku":"FY12366","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_638cd816-b707-4f01-b13f-2da99f4555de.jpg?v=1782236995","url":"https:\/\/www.ebiohippo.com\/products\/pbx4-antibody-pre-b-cell-leukemia-transcription-factor-4-bha17135269","provider":"BioHippo","version":"1.0","type":"link"}