{"product_id":"pcbd1-antibody-pterin-4-alpha-carbinolamine-dehydratase-bha17135692","title":"PCBD1 Antibody \/ Pterin-4 alpha-carbinolamine dehydratase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePCBD1 Antibody \/ Pterin-4 alpha-carbinolamine dehydratase is a anti-PCBD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PCBD1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePCBD1 antibody detects Pterin-4 alpha-carbinolamine dehydratase, a bifunctional enzyme that plays a role in tetrahydrobiopterin (BH4) regeneration and acts as a dimerization cofactor for hepatocyte nuclear factor 1 alpha (HNF1A). Encoded by the PCBD1 gene on chromosome 10q22.1, this protein contributes to phenylalanine, tyrosine, and tryptophan hydroxylation by recycling BH4, an essential cofactor for aromatic amino acid hydroxylases. PCBD1 thereby supports neurotransmitter biosynthesis and amino acid metabolism while also functioning in transcriptional regulation.\u003cbr\u003e\u003cbr\u003eIn its enzymatic role, PCBD1 catalyzes the dehydration of pterin-4 alpha-carbinolamine to quinonoid dihydrobiopterin, a step required for BH4 cofactor regeneration. Independently, as a dimerization cofactor, it stabilizes the DNA-binding form of HNF1A, influencing transcription of genes involved in glucose metabolism and hepatic differentiation. This dual functionality links metabolic and transcriptional control mechanisms within hepatocytes, endocrine tissues, and the central nervous system.\u003cbr\u003e\u003cbr\u003eThe PCBD1 antibody is widely used in enzymology, metabolic, and transcriptional biology research to study BH4 metabolism, amino acid hydroxylation, and HNF1A-dependent transcription. Western blot analysis identifies a 12 kilodalton band corresponding to PCBD1, while immunofluorescence reveals cytoplasmic and nuclear localization depending on cellular context. This antibody supports the investigation of enzyme regulation and transcriptional cofactor dynamics.\u003cbr\u003e\u003cbr\u003eMutations in PCBD1 cause hyperphenylalaninemia and transient neonatal diabetes due to impaired cofactor recycling and altered HNF1A transcriptional activity. The PCBD1 antibody is an essential reagent for exploring metabolic diseases, neurotransmitter synthesis, and transcriptional regulation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PCBD1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PCBD1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PCBD1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PCBD1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PCBD1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047301931373,"sku":"FY12790","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_5274b752-26a5-43f8-b068-778bf0457991.jpg?v=1782237046","url":"https:\/\/www.ebiohippo.com\/products\/pcbd1-antibody-pterin-4-alpha-carbinolamine-dehydratase-bha17135692","provider":"BioHippo","version":"1.0","type":"link"}