{"product_id":"pcdha2-antibody-protocadherin-alpha-2-bha17136279","title":"PCDHA2 Antibody \/ Protocadherin alpha 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePCDHA2 Antibody \/ Protocadherin alpha 2 is a anti-PCDHA2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PCDHA2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePCDHA2 antibody detects Protocadherin alpha 2, a calcium-dependent cell adhesion protein encoded by the PCDHA2 gene on chromosome 5q31.3. PCDHA2 belongs to the protocadherin alpha gene cluster, a group of neural adhesion molecules that mediate cell-cell recognition, axon targeting, and synaptic specificity in the central nervous system. It is expressed predominantly in neurons, where it contributes to the establishment and maintenance of precise neural connectivity patterns. PCDHA2 plays an essential role in neuronal identity and self-avoidance through its combinatorial expression with other protocadherins in the alpha cluster.\u003cbr\u003e\u003cbr\u003eStructurally, PCDHA2 is a single-pass transmembrane glycoprotein composed of six extracellular cadherin repeats, a transmembrane domain, and a conserved cytoplasmic tail that interacts with intracellular signaling molecules. It belongs to the cadherin superfamily, which includes classical cadherins, desmogleins, and other protocadherin clusters (beta and gamma). The extracellular cadherin domains of PCDHA2 mediate homophilic adhesion through calcium-dependent binding, while the cytoplasmic region connects to the actin cytoskeleton via catenins and other scaffold proteins.\u003cbr\u003e\u003cbr\u003eFunctionally, PCDHA2 promotes cell adhesion specificity during neuronal development. It participates in establishing unique neuronal surface codes that guide synaptic pairing and axonal targeting. In mature neurons, PCDHA2 contributes to dendritic self-avoidance, ensuring that neurites from the same cell do not form redundant connections. It also supports synaptic stability by maintaining adhesion at postsynaptic densities. Co-localization studies show PCDHA2 localized to dendritic membranes and synaptic junctions, where it interacts with cytoskeletal and signaling partners.\u003cbr\u003e\u003cbr\u003ePCDHA2 expression is developmentally regulated, with high levels during neural differentiation and synapse formation. Dysregulation or mutation of protocadherin alpha genes, including PCDHA2, has been associated with autism spectrum disorder, epilepsy, and schizophrenia, highlighting their importance in brain circuit organization. Pathway involvement includes cell adhesion, synaptic signaling, and calcium-dependent cell communication. Isoform diversity among protocadherin alpha genes allows neurons to generate individual identity codes critical for neural wiring precision.\u003cbr\u003e\u003cbr\u003eThe PCDHA2 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PCDHA2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PCDHA2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PCDHA2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PCDHA2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PCDHA2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047323787629,"sku":"FY13377","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_2877aed7-2af6-4875-8034-48668c93960a.jpg?v=1782237106","url":"https:\/\/www.ebiohippo.com\/products\/pcdha2-antibody-protocadherin-alpha-2-bha17136279","provider":"BioHippo","version":"1.0","type":"link"}