{"product_id":"pcgf6-antibody-polycomb-group-ring-finger-protein-6-bha17135674","title":"PCGF6 Antibody \/ Polycomb group RING finger protein 6","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePCGF6 Antibody \/ Polycomb group RING finger protein 6 is a anti-PCGF6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PCGF6\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePCGF6 antibody detects Polycomb group RING finger protein 6, a transcriptional repressor that functions as part of noncanonical Polycomb repressive complex 1.6 (PRC1.6). Encoded by the PCGF6 gene on chromosome 10p15.3, this protein belongs to the Polycomb group (PcG) family, which maintains transcriptional repression of developmental and cell cycle genes through histone modification and chromatin compaction. PCGF6 acts as a key scaffold within PRC1.6, recruiting additional subunits including L3MBTL2, RING1A\/B, and E2F6, to mediate histone H2A monoubiquitination and gene silencing independent of canonical PRC1 complexes.\u003cbr\u003e\u003cbr\u003ePCGF6 contains a RING finger domain responsible for interacting with the ubiquitin ligases RING1A\/B, promoting histone modification that represses gene transcription. Through association with the transcriptional repressors E2F6 and MGA, PCGF6 regulates genes involved in cell proliferation, germline maintenance, and differentiation. It is expressed in embryonic stem cells, germ cells, and certain somatic tissues, where it contributes to pluripotency and developmental gene silencing. PCGF6 activity ensures appropriate repression of lineage-specific genes during embryogenesis and germ cell development.\u003cbr\u003e\u003cbr\u003eThe PCGF6 antibody is used in epigenetics, developmental biology, and transcriptional regulation studies to investigate Polycomb complex function and chromatin remodeling. Western blot analysis detects a 37 kilodalton band corresponding to PCGF6, and immunofluorescence reveals nuclear punctate staining associated with chromatin regions. This antibody enables researchers to track PRC1.6 distribution and assess its role in transcriptional repression and stem cell identity.\u003cbr\u003e\u003cbr\u003eAberrant expression of PCGF6 has been implicated in cancer and developmental disorders. Overexpression supports cell proliferation and transformation, while depletion can impair germ cell specification and induce differentiation. By regulating histone ubiquitination and chromatin architecture, PCGF6 acts as a molecular link between epigenetic control and transcriptional silencing. The PCGF6 antibody provides a dependable tool for studying these mechanisms in both stem cell and cancer research.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PCGF6 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PCGF6 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PCGF6 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PCGF6 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PCGF6+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047299539309,"sku":"FY12771","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_52a558dd-ab7c-46e8-a23e-f1d34653e3de.jpg?v=1782237043","url":"https:\/\/www.ebiohippo.com\/products\/pcgf6-antibody-polycomb-group-ring-finger-protein-6-bha17135674","provider":"BioHippo","version":"1.0","type":"link"}