{"product_id":"pcif1-antibody-phosphorylated-ctd-interacting-factor-1-bha17135851","title":"PCIF1 Antibody \/ Phosphorylated CTD-interacting factor 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePCIF1 Antibody \/ Phosphorylated CTD-interacting factor 1 is a anti-PCIF1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PCIF1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePCIF1 antibody detects Phosphorylated CTD-interacting factor 1, an mRNA cap-specific methyltransferase that modifies the first transcribed nucleotide at the 5' cap structure of mRNA. The UniProt recommended name is mRNA cap-specific adenosine N6 methyltransferase (PCIF1), also known as CAPAM or phosphorylated CTD-interacting factor 1. This enzyme catalyzes the methylation of N6-adenosine in the cap-adjacent nucleotide, creating the m6Am modification, a key regulator of mRNA stability and translation efficiency.\u003cbr\u003e\u003cbr\u003eFunctionally, PCIF1 antibody identifies a 726-amino-acid nuclear protein that directly interacts with the C-terminal domain (CTD) of RNA polymerase II when it is phosphorylated on Ser5 residues. This interaction positions PCIF1 near nascent transcripts to catalyze co-transcriptional methylation. The resulting m6Am mark enhances mRNA translation efficiency and protects transcripts from decapping and degradation. Through this function, PCIF1 plays a central role in post-transcriptional gene regulation and RNA processing.\u003cbr\u003e\u003cbr\u003eThe PCIF1 gene is located on chromosome 20q13.13 and encodes a methyltransferase that contains a SAM-dependent catalytic domain typical of N6-adenosine methyltransferases. Its activity is tightly linked to transcriptional elongation and is coordinated with other cap modifications such as m7G capping and 2'-O-methylation. PCIF1 also associates with components of the mRNA export machinery, suggesting a coupling between RNA methylation and nuclear export.\u003cbr\u003e\u003cbr\u003eLoss or dysregulation of PCIF1 affects global mRNA methylation profiles, leading to alterations in gene expression, differentiation, and stress response. Studies indicate that depletion of PCIF1 reduces m6Am abundance and decreases translation of specific mRNAs, particularly those involved in growth control and stress adaptation. Conversely, overexpression enhances translation of transcripts with cap-proximal adenosines, promoting cell survival under adverse conditions.\u003cbr\u003e\u003cbr\u003ePCIF1 antibody is widely used in research to examine RNA modification pathways, cap-binding protein interactions, and transcriptional regulation. It is valuable for immunoblotting, immunofluorescence, and co-immunoprecipitation assays to identify PCIF1 localization and activity. In cancer studies, aberrant PCIF1 expression correlates with altered methylation landscapes and tumor progression. PCIF1 has also been linked to viral RNA modification, influencing immune detection of viral transcripts.\u003cbr\u003e\u003cbr\u003eThrough its role in mRNA cap regulation, PCIF1 coordinates multiple levels of gene expression control from transcription initiation to translation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PCIF1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PCIF1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PCIF1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PCIF1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PCIF1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047306649965,"sku":"FY12949","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_78f1939f-aecd-478e-b43f-f408bcee1c84.jpg?v=1782237059","url":"https:\/\/www.ebiohippo.com\/products\/pcif1-antibody-phosphorylated-ctd-interacting-factor-1-bha17135851","provider":"BioHippo","version":"1.0","type":"link"}