{"product_id":"pcp2-antibody-purkinje-cell-protein-2-bha17135714","title":"PCP2 Antibody \/ Purkinje cell protein 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePCP2 Antibody \/ Purkinje cell protein 2 is a anti-PCP2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PCP2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePCP2 antibody detects Purkinje cell protein 2, a brain-specific regulator of G protein signaling and neuronal function. Encoded by the PCP2 gene on chromosome 19p13.2, this protein is selectively expressed in cerebellar Purkinje cells and retinal bipolar neurons. PCP2, also known as L7, modulates intracellular signaling by binding to Galpha subunits of heterotrimeric G proteins, influencing synaptic transmission and motor coordination.\u003cbr\u003e\u003cbr\u003ePCP2 contains a GoLoco motif that enables high-affinity binding to inactive GDP-bound Galpha subunits, acting as a guanine nucleotide dissociation inhibitor (GDI). Through this interaction, it regulates G protein-coupled receptor (GPCR) signaling pathways that affect neuronal excitability and neurotransmitter release. PCP2 expression increases during cerebellar development, coinciding with synapse formation and maturation of Purkinje cells.\u003cbr\u003e\u003cbr\u003eThe PCP2 antibody is widely used in neurobiology, developmental, and signaling research to investigate cerebellar function, G protein regulation, and neuronal connectivity. Western blot analysis identifies a 17 kilodalton band corresponding to PCP2, while immunohistochemistry shows intense staining in Purkinje cell somata and dendrites, as well as retinal bipolar neurons. This antibody enables precise visualization of neuronal subtypes and signaling dynamics in the cerebellum and retina.\u003cbr\u003e\u003cbr\u003eAltered expression of PCP2 has been associated with ataxia, autism spectrum disorders, and visual processing abnormalities, reflecting its role in synaptic signal modulation. The PCP2 antibody provides a sensitive tool for exploring cerebellar physiology and G protein regulation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PCP2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PCP2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PCP2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PCP2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PCP2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047302717805,"sku":"FY12812","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f2f5c4a1-aadb-457c-b555-85f781d4c517.jpg?v=1782237044","url":"https:\/\/www.ebiohippo.com\/products\/pcp2-antibody-purkinje-cell-protein-2-bha17135714","provider":"BioHippo","version":"1.0","type":"link"}