{"product_id":"pcr-circle-purification-kit-bht15200006","title":"PCR Circle Purification Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eDNA Purification Kits\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThis PCR product and plasmid clean-up kit recovers high-purity DNA fragments containing no salt or low salt and no impurities such as protein or RNA from the PCR reaction system. The 200 bp–10 kb DNA fragment has a recovery rate of more than 80%, and can recover single-stranded, double-stranded DNA fragments and circular plasmid DNA. Recovered DNA can be used directly in restriction digestion, ligation and sequencing reactions.\u003c\/p\u003e\n\u003cp\u003eThe PCR product is mixed with five volumes of Solution PG, or six volumes for fragments under 500 bp, and loaded onto Adsorption column G. The column is washed twice with 600 µL of Wash Buffer, centrifuged at 13,000 × g for 2 min, air-dried for 2–4 min and eluted with 15–50 µL of Elution Buffer, with 30 µL recommended. Recovered DNA has an OD260\/OD280 of about 1.8–2.0.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eSpin column (Adsorption column G)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample type\u003c\/th\u003e\n\u003ctd\u003ePCR reaction products; single-stranded, double-stranded and circular plasmid DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input\u003c\/th\u003e\n\u003ctd\u003ePCR reaction below 300 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFragment range\u003c\/th\u003e\n\u003ctd\u003e200 bp–10 kb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRecovery\u003c\/th\u003e\n\u003ctd\u003eMore than 80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBinding capacity\u003c\/th\u003e\n\u003ctd\u003eUp to 10 µg DNA per column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003eOD260\/OD280 is about 1.8–2.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution volume\u003c\/th\u003e\n\u003ctd\u003e15–50 µL, 30 µL recommended\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing time\u003c\/th\u003e\n\u003ctd\u003e15 min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e50 T\u003c\/th\u003e\n\u003cth\u003e200 T\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolution PG\u003c\/td\u003e\n\u003ctd\u003e20 mL\u003c\/td\u003e\n\u003ctd\u003e80 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer\u003c\/td\u003e\n\u003ctd\u003e60 mL\u003c\/td\u003e\n\u003ctd\u003e240 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eElution Buffer\u003c\/td\u003e\n\u003ctd\u003e5 mL\u003c\/td\u003e\n\u003ctd\u003e20 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdsorption column G\u003c\/td\u003e\n\u003ctd\u003e50 set\u003c\/td\u003e\n\u003ctd\u003e200 set\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePurification of PCR products\u003c\/li\u003e\n\u003cli\u003eRecovery of circular plasmid DNA\u003c\/li\u003e\n\u003cli\u003eEnzyme digestion and ligation of recovered fragments\u003c\/li\u003e\n\u003cli\u003eSequencing reactions\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eFast speed: 15 minutes to complete DNA recovery, can process multiple samples at the same time, save time.\u003c\/li\u003e\n\u003cli\u003eFewer steps: Simple operation — the purification is completed in a few centrifugation steps.\u003c\/li\u003e\n\u003cli\u003eHigh efficiency: The unique spin column and carefully prepared buffer can recover a large amount of high purity target DNA each time.\u003c\/li\u003e\n\u003cli\u003eProcessing capacity: The amount of DNA that can be adsorbed by each centrifugal adsorption column each time is up to 10 µg.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eAll components are stored at room temperature for 1 year.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCheck that the pH of Solution PG is 7.5 or below after opening.\u003c\/li\u003e\n\u003cli\u003eAdd ethanol to the Wash Buffer before first use.\u003c\/li\u003e\n\u003cli\u003eKeep the PCR reaction volume below 300 µL.\u003c\/li\u003e\n\u003cli\u003eUse six volumes of Solution PG for fragments under 500 bp.\u003c\/li\u003e\n\u003cli\u003eAir-dry the column for 2–4 min before elution; residual ethanol lowers recovery.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEP005\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200006\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"200 T","offer_id":53965511983469,"sku":"EP005-200T","price":208.0,"currency_code":"USD","in_stock":true},{"title":"50 T x 10","offer_id":53965514441069,"sku":"EP005-50TX10","price":0.0,"currency_code":"USD","in_stock":true},{"title":"200 T x 5","offer_id":53965514473837,"sku":"EP005-200TX5","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EP005-a-1.jpg?v=1789419190","url":"https:\/\/www.ebiohippo.com\/products\/pcr-circle-purification-kit-bht15200006","provider":"BioHippo","version":"1.0","type":"link"}