{"product_id":"pcyox1-antibody-prenylcysteine-oxidase-1-bha17135800","title":"PCYOX1 Antibody \/ Prenylcysteine oxidase 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePCYOX1 Antibody \/ Prenylcysteine oxidase 1 is a anti-PCYOX1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm (lysosome).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PCYOX1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePCYOX1 antibody detects Prenylcysteine oxidase 1, an enzyme that catalyzes the final step in the degradation of prenylated proteins. Encoded by the PCYOX1 gene on chromosome 2p21, this flavin-dependent oxidase converts prenylcysteine residues-generated from protein turnover-into cysteine sulfinic acid, carbon dioxide, and prenyl aldehydes. PCYOX1 thereby contributes to the detoxification and recycling of lipid-modified proteins and regulates cellular redox balance.\u003cbr\u003e\u003cbr\u003eStructurally, PCYOX1 is a 501-amino-acid enzyme of approximately 57 kilodaltons containing a flavin adenine dinucleotide (FAD)-binding domain responsible for oxidative catalysis. It localizes to the endoplasmic reticulum and cytosol, where it associates with membranes to metabolize prenylated cysteine derivatives derived from small GTPases such as Ras and Rho. This activity maintains proper turnover of prenylated signaling proteins and limits accumulation of reactive intermediates that could disrupt cell membranes.\u003cbr\u003e\u003cbr\u003eThe PCYOX1 antibody is widely used in metabolism, enzymology, and redox biology research to study lipid modification turnover, oxidative regulation, and prenylated protein catabolism. Western blot analysis detects a 57 kilodalton band corresponding to PCYOX1, while immunofluorescence shows cytosolic and reticular staining in metabolically active tissues such as liver and kidney. This antibody provides a reliable tool for assessing enzyme function in lipid processing and oxidative homeostasis.\u003cbr\u003e\u003cbr\u003eFunctionally, PCYOX1 acts downstream of protein prenylation pathways that modify small GTPases for membrane targeting and signal transduction. By degrading prenylcysteine products, it prevents the accumulation of toxic intermediates and contributes to lipid-derived aldehyde metabolism. PCYOX1 activity is linked to oxidative stress response, with increased expression observed in conditions of lipid peroxidation and metabolic overload. Dysregulation of PCYOX1 expression has been associated with atherosclerosis and metabolic disease, implicating its role in vascular oxidative stress. The PCYOX1 antibody provides a valuable reagent for studying the interplay between protein modification, lipid metabolism, and oxidative signaling.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PCYOX1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PCYOX1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PCYOX1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PCYOX1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PCYOX1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047305765229,"sku":"FY12898","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_982cb2f5-c12d-44f1-932c-370821a8b6df.jpg?v=1782237060","url":"https:\/\/www.ebiohippo.com\/products\/pcyox1-antibody-prenylcysteine-oxidase-1-bha17135800","provider":"BioHippo","version":"1.0","type":"link"}