{"product_id":"pcyt2-antibody-ethanolamine-phosphate-cytidylyltransferase-bha17135398","title":"PCYT2 Antibody \/ Ethanolamine-phosphate cytidylyltransferase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePCYT2 Antibody \/ Ethanolamine-phosphate cytidylyltransferase is a anti-PCYT2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PCYT2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePCYT2 antibody detects Ethanolamine-phosphate cytidylyltransferase, a key enzyme in the CDP-ethanolamine branch of the Kennedy pathway for phosphatidylethanolamine synthesis. PCYT2 catalyzes the formation of CDP-ethanolamine from CTP and phosphoethanolamine, an essential step in the biosynthesis of membrane phospholipids. The PCYT2 antibody is used in lipid metabolism, neurobiology, and mitochondrial research to study phospholipid homeostasis and membrane biogenesis.\u003cbr\u003e\u003cbr\u003ePCYT2 is encoded by the PCYT2 gene located on human chromosome 17q25.3. The protein is approximately 43 kilodaltons and localized predominantly in the endoplasmic reticulum. It functions as a rate-limiting enzyme that determines phosphatidylethanolamine synthesis levels, thereby influencing membrane fluidity, mitochondrial morphology, and cellular signaling. PCYT2 activity is critical for maintaining the structural integrity of cell membranes and organelles, particularly in the nervous system and muscle tissue.\u003cbr\u003e\u003cbr\u003eUsing the PCYT2 antibody, researchers can identify the 43 kilodalton protein by western blot and immunofluorescence, where it shows perinuclear localization corresponding to the ER network. Genetic and biochemical studies have shown that PCYT2 deficiency results in reduced phosphatidylethanolamine production, leading to mitochondrial dysfunction, abnormal lipid droplet accumulation, and neurodevelopmental impairment. Mutations in PCYT2 cause hereditary spastic paraplegia type 82 and neuromuscular lipid metabolism disorders.\u003cbr\u003e\u003cbr\u003ePCYT2 expression is regulated by nutritional and hormonal cues, including insulin and fatty acid levels. It interacts with lipid transfer proteins and participates in the coordination between the Kennedy pathway and mitochondrial phospholipid synthesis. \u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PCYT2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PCYT2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PCYT2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PCYT2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PCYT2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047290986861,"sku":"FY12495","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b5d44add-3754-4563-bce8-7389e291b4f0.jpg?v=1782237012","url":"https:\/\/www.ebiohippo.com\/products\/pcyt2-antibody-ethanolamine-phosphate-cytidylyltransferase-bha17135398","provider":"BioHippo","version":"1.0","type":"link"}