{"product_id":"pd1-cd28-nfat-reporter-lentivirus-bhv19400255","title":"PD1-CD28\/NFAT Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ePD-1 (PDCD1) is a co-inhibitory receptor on activated T cells that, upon engaging its ligands PD-L1 and PD-L2, dampens T cell receptor signaling and promotes peripheral tolerance and tumor immune evasion. CD28 is the principal co-stimulatory receptor that, by binding CD80 and CD86, amplifies T cell activation, proliferation, and survival. Engineered PD1-CD28 switch receptors fuse the PD-1 ectodomain to the CD28 signaling domain so that an inhibitory input is converted into a stimulatory one. Both signals converge on NFAT, a calcium-responsive transcription factor that drives effector gene expression downstream of the T cell receptor, making NFAT-based reporters a quantitative measure of T cell activation in checkpoint biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe PD1-CD28\/NFAT Reporter Lentivirus is a two-component immunotherapy reporter system. A receptor construct uses an EF1a promoter to constitutively co-express human PD1 and CD28, separated by a P2A linker, together with a fluorescent reporter or drug selection marker for stable cell line selection. A separate cassette drives a reporter through tandem NFAT response elements coupled to a minimal promoter and upstream enhancer, yielding a fluorescent (GFP or RFP) and\/or secreted Gaussia luciferase readout of T cell activity.\u003c\/p\u003e\n\u003cp\u003eSequential transduction generates dual-stable effector lines for studying PD-1 pathway modulation, checkpoint-blockade agents, and switch-receptor engineering. Supplied as high-titer, VSV-G-pseudotyped particles purified by PEG precipitation and sucrose gradient centrifugation, suitable for primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human PD1-P2A-CD28 under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"RFP \/ GFP (constitutively expressed) \/ 2x10^6","offer_id":53251622732141,"sku":"TRV-0005-1S","price":895.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ RFP (constitutively expressed) \/ 2x10^6","offer_id":53362029953389,"sku":"TRV-0005-2S","price":895.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 2x10^6","offer_id":53362029986157,"sku":"TRV-0005-3S","price":895.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 2x10^6","offer_id":53362030018925,"sku":"TRV-0005-4S","price":895.0,"currency_code":"USD","in_stock":true},{"title":"GFP-P2A-GLuc \/ Puromycin \/ 1x10^6","offer_id":53362030051693,"sku":"TRV-0005-5S","price":895.0,"currency_code":"USD","in_stock":true},{"title":"GFP-P2A-GLuc \/ Blasticidin \/ 2x10^6","offer_id":53362030084461,"sku":"TRV-0005-6S","price":895.0,"currency_code":"USD","in_stock":true},{"title":"RFP-P2A-GLuc \/ Puromycin \/ 1x10^6","offer_id":53362030117229,"sku":"TRV-0005-7S","price":895.0,"currency_code":"USD","in_stock":true},{"title":"RFP-P2A-GLuc \/ Blasticidin \/ 2x10^6","offer_id":53362030149997,"sku":"TRV-0005-8S","price":895.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Vector-Layout-1024x261_3d4c98dc-04b9-4983-84e9-0b1fbf2721a4.jpg?v=1782157779","url":"https:\/\/www.ebiohippo.com\/products\/pd1-cd28-nfat-reporter-lentivirus-bhv19400255","provider":"BioHippo","version":"1.0","type":"link"}