{"product_id":"pde7a-creb-reporter-lentivirus-bhv19400271","title":"PDE7A\/CREB Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ePDE7A is a cAMP-specific phosphodiesterase that hydrolyzes the second messenger cyclic AMP and thereby limits signaling through the cAMP\/PKA\/CREB pathway. CREB (cAMP response element-binding protein) is a transcription factor that, when phosphorylated downstream of elevated cAMP, binds cAMP response elements to activate target genes. In T lymphocytes, cAMP levels influence activation and proliferation, and PDE7A helps set the threshold for these responses by controlling cAMP turnover. Because of this role, PDE7A is studied as a regulator of immune cell function and as a potential drug target, and CREB-driven reporters provide a quantitative readout of how PDE7A activity shapes cAMP-dependent transcription.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe PDE7A\/CREB Reporter Lentivirus is an immunotherapy reporter system for studying PDE7A function within the cAMP\/CREB pathway. A receptor lentivirus constitutively expresses human PDE7A with antibiotic selection, while a reporter lentivirus carries CREB response elements driving a dual reporter, secreted Gaussia luciferase and a fluorescent protein. Sequential transduction and selection generates a dual-stable cell line in which PDE7A activity modulates intracellular cAMP and the resulting CREB-dependent reporter output.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase accumulates in conditioned media, enabling kinetic sampling without cell lysis, while fluorescence supports microscopy and flow cytometry. The VSV-G-pseudotyped particles are purified by PEG precipitation and sucrose gradient centrifugation and transduce difficult-to-transfect cells, including primary and thawed cultures, supporting T-cell activation assays and immune signaling research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human PDE7A under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GLuc-P2A-GFP \/ Blasticidin \/ 2x10^6","offer_id":53251623289197,"sku":"TRV-0023-6S","price":895.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Vector-Layout-1024x261_ed8ed085-8e65-4a34-8fe9-8d9fe65dc00a.jpg?v=1782157779","url":"https:\/\/www.ebiohippo.com\/products\/pde7a-creb-reporter-lentivirus-bhv19400271","provider":"BioHippo","version":"1.0","type":"link"}