{"product_id":"pde8a-antibody-phosphodiesterase-8a-bha17135447","title":"PDE8A Antibody \/ Phosphodiesterase 8A","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePDE8A Antibody \/ Phosphodiesterase 8A is a anti-PDE8A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PDE8A\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePDE8A antibody detects Phosphodiesterase 8A, an enzyme that specifically hydrolyzes cyclic adenosine monophosphate (cAMP) to AMP, thereby regulating intracellular signaling pathways mediated by this second messenger. PDE8A belongs to the type 8 family of cyclic nucleotide phosphodiesterases, which are distinct in their insensitivity to common PDE inhibitors such as IBMX. The PDE8A antibody is widely used in studies of hormonal regulation, metabolism, and immune signaling, where precise modulation of cAMP levels is critical.\u003cbr\u003e\u003cbr\u003ePDE8A is encoded by the PDE8A gene located on human chromosome 15q25.3. The enzyme is approximately 93 kilodaltons and contains a conserved catalytic domain flanked by unique N-terminal and C-terminal regulatory regions. PDE8A is expressed in various tissues, including adrenal gland, heart, testis, and immune cells, where it contributes to compartmentalized cAMP degradation and pathway specificity. Its high substrate affinity and resistance to standard inhibitors make it a key regulator of localized signaling domains.\u003cbr\u003e\u003cbr\u003eThe PDE8A antibody detects multiple isoforms ranging from 85-100 kilodaltons on western blot and reveals cytoplasmic and perinuclear staining in immunofluorescence assays. PDE8A associates with A-kinase anchoring proteins (AKAPs) and participates in cAMP microdomain organization, influencing downstream activation of protein kinase A (PKA) and exchange proteins activated by cAMP (EPACs). In immune cells, PDE8A modulates T-cell receptor signaling and cytokine production, contributing to immune tolerance and inflammatory regulation.\u003cbr\u003e\u003cbr\u003eFunctional studies indicate that PDE8A influences steroidogenesis, insulin secretion, and energy balance by controlling cAMP-mediated transcriptional events. Knockdown or pharmacological inhibition enhances cAMP signaling and affects processes such as sperm motility, cortisol synthesis, and cardiac contractility. Altered PDE8A expression has been linked to autoimmune diseases, infertility, and metabolic disorders.\u003cbr\u003e\u003cbr\u003ePDE8A also plays roles in cancer biology, where its dysregulation affects cell proliferation and migration through altered PKA activity and transcriptional responses.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PDE8A - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PDE8A - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PDE8A - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PDE8A - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PDE8A+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047292232045,"sku":"FY12544","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_33d71279-ba65-49c7-90f6-d234bcae4ea2.jpg?v=1782237010","url":"https:\/\/www.ebiohippo.com\/products\/pde8a-antibody-phosphodiesterase-8a-bha17135447","provider":"BioHippo","version":"1.0","type":"link"}