{"product_id":"pdgfd-orf-cdna-lentivirus-bhv19400098","title":"PDGFD ORF cDNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ePDGF-D (platelet-derived growth factor D) is a member of the PDGF family of growth factors. It is secreted as a latent protein that requires proteolytic removal of its CUB domain to release the active growth-factor domain, which then signals primarily through the PDGF receptor beta. PDGF-D signaling stimulates the proliferation, migration, and survival of mesenchymal cells such as fibroblasts and smooth muscle cells, and contributes to angiogenesis, wound healing, and tissue remodeling. Aberrant PDGF-D activity has been linked to fibrosis, atherosclerosis, and tumor progression, where it can promote a supportive microenvironment, making PDGF-D a target of interest in cancer and cell biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe PDGFD ORF cDNA Lentivirus delivers the open reading frame of human PDGF-D (transcript variant 1) for stable overexpression in mammalian cells. A CMV promoter drives expression of PDGF-D fused to a C-terminal V5 tag, with a fluorescent reporter (GFP or RFP) and a drug selection marker (puromycin or blasticidin) separated from the cDNA by self-cleaving peptides for independent translation. In some configurations a PGK promoter drives the reporter and selection marker.\u003c\/p\u003e\n\u003cp\u003eThe vectors are optimized for high expression and reliable genome integration, enabling long-term stable cell lines. ORF cloning accuracy is confirmed by sequencing and protein expression validated by transient transfection. Particles efficiently transduce difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis ORF cDNA lentivirus enables stable overexpression of PDGFD (NCBI Accession: NM_025208) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (GFP, RFP; Puromycin, Blasticidin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.\u003c\/p\u003e\n\u003cp\u003eUltra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"CMV-PDGFD-V5\/GFP \/ RFP \/ 5x10^6","offer_id":53251623059821,"sku":"LCV-0003-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-PDGFD-V5\/RFP \/ RFP \/ 5x10^6","offer_id":53311264424301,"sku":"LCV-0003-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-PDGFD-Flag\/Puro \/ N\/A \/ 5x10^6","offer_id":53311264457069,"sku":"LCV-0003-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-PDGFD-Flag\/BSD \/ N\/A \/ 5x10^6","offer_id":53311264489837,"sku":"LCV-0003-4S","price":595.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/pdgfd-orf-cdna-lentivirus-bhv19400098","provider":"BioHippo","version":"1.0","type":"link"}