{"product_id":"pdpr-antibody-pyruvate-dehydrogenase-phosphatase-regulatory-subunit-bha17136243","title":"PDPR Antibody \/ Pyruvate dehydrogenase phosphatase regulatory subunit","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePDPR Antibody \/ Pyruvate dehydrogenase phosphatase regulatory subunit is a anti-PDPR Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PDPR\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePDPR antibody detects Pyruvate dehydrogenase phosphatase regulatory subunit, a mitochondrial matrix protein encoded by the PDPR gene located on chromosome 16q22.1. PDPR acts as a regulatory subunit of the pyruvate dehydrogenase phosphatase (PDP) complex, which controls activity of the pyruvate dehydrogenase complex (PDC) - a critical enzyme in carbohydrate metabolism that links glycolysis to the tricarboxylic acid (TCA) cycle. PDPR is highly expressed in metabolically active tissues such as liver, heart, skeletal muscle, and kidney, where it modulates energy production and metabolic flexibility.\u003cbr\u003e\u003cbr\u003ePDPR functions by modulating the catalytic activity of PDP1 and PDP2 phosphatase subunits, facilitating dephosphorylation and reactivation of PDC. This regulation ensures efficient conversion of pyruvate to acetyl-CoA, thereby supporting ATP generation under nutrient-rich conditions. PDPR also influences glucose oxidation rates and metabolic adaptation during fasting, exercise, or insulin stimulation. Co-localization studies show PDPR associating with PDC and PDP1 in the mitochondrial matrix, maintaining dynamic control over carbohydrate flux.\u003cbr\u003e\u003cbr\u003eStructurally, PDPR contains an N-terminal domain involved in protein-protein interactions and a C-terminal alpha-helical region required for complex stability. It belongs to the pyruvate dehydrogenase regulatory protein family, which fine-tunes mitochondrial energy metabolism. The protein�s structural motifs enable binding with PDP catalytic subunits and coordination with mitochondrial targeting sequences for correct localization.\u003cbr\u003e\u003cbr\u003eFunctionally, PDPR integrates metabolic and hormonal cues to maintain energy homeostasis. It plays a vital role in glucose utilization, mitochondrial respiration, and lipid oxidation balance. PDPR is regulated by insulin, glucagon, and nutrient availability, allowing cells to rapidly switch between carbohydrate and fatty acid metabolism. During embryonic development, PDPR supports energy demands of rapidly growing tissues, particularly cardiac and skeletal muscle. Pathway involvement includes the TCA cycle, oxidative phosphorylation, and insulin signaling pathways that coordinate energy metabolism.\u003cbr\u003e\u003cbr\u003eDysregulation of PDPR activity is linked to metabolic diseases, including diabetes, obesity, and mitochondrial disorders. Reduced PDPR function leads to impaired PDC activation, resulting in accumulation of pyruvate and lactic acid, while overactivation can alter glucose oxidation balance. Genetic variants in PDPR have been associated with altered fasting glucose levels and insulin sensitivity. Because of its role in mitochondrial regulation, PDPR has also been studied in cancer metabolism, where metabolic reprogramming supports tumor cell proliferation.\u003cbr\u003e\u003cbr\u003eImmunohistochemical staining using PDPR antibody shows mitochondrial localization in liver, cardiac, and muscle cells. The PDPR antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PDPR - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PDPR - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PDPR - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PDPR - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PDPR+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047322706285,"sku":"FY13341","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_76004deb-37fa-4021-a7b3-f704dc693308.jpg?v=1782237107","url":"https:\/\/www.ebiohippo.com\/products\/pdpr-antibody-pyruvate-dehydrogenase-phosphatase-regulatory-subunit-bha17136243","provider":"BioHippo","version":"1.0","type":"link"}