{"product_id":"pdx1-recombinant-monoclonal-antibody-bha10576579","title":"PDX1 Recombinant Monoclonal Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis is a monoclonal anti-PDX1 antibody raised in Rabbit, with confirmed utility in ELISA, WB, IHC, FC. It is designed to detect PDX1 protein in Human and supports researchers working in others contexts.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e A synthesized peptide from human PDX1 protein — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary reagents for indirect detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMonoclonal format:\u003c\/strong\u003e Single-epitope binding provides high specificity and lot-to-lot reproducibility. Confirm epitope accessibility under your assay conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Matched secondary antibodies and isotype controls required. Compatible with standard Protein A\/G purification workflows.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Affinity chromatography):\u003c\/strong\u003e Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePDX1\u003c\/strong\u003e (also referred to as Glucose sensitive factor antibody, Glucose-sensitive factor antibody, GSF antibody, IDX 1 antibody, IDX-1 antibody, IDX1 antibody, Insulin promoter factor 1 antibody, Insulin promoter factor 1 homeodomain transcription factor antibody, Insulin upstream factor 1 antibody, IPF 1 antibody, IPF-1 antibody, IPF1 antibody, Islet\/duodenum homeobox 1 antibody, Islet\/duodenum homeobox-1 antibody, IUF 1 antibody, IUF-1 antibody, IUF1 antibody, MODY4 antibody, Pancreas\/duodenum homeobox 1 antibody, Pancreas\/duodenum homeobox protein 1 antibody, pancreatic and duodenal homeobox P antibody, PDX 1 antibody, PDX-1 antibody, PDX1 antibody, PDX1_HUMAN antibody, Somatostatin transactivating factor 1 antibody, Somatostatin-transactivating factor 1 antibody, STF 1 antibody, STF-1 antibody, STF1 antibody) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of PDX1 biology in others.\u003c\/p\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWB:\u003c\/strong\u003e Confirms target molecular weight and distinguishes isoforms under denaturing conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIHC:\u003c\/strong\u003e Visualization of target in FFPE or frozen tissue sections. Optimize antigen retrieval and secondary detection for each tissue type.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFC:\u003c\/strong\u003e Applicable for FC-based detection; refer to product documentation for optimized conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Confirm the clone does not cross-react with related family members under your assay conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Confirmed for Human. Extrapolation to untested species requires empirical validation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cem\u003eThis product is a recombinant monoclonal antibody expressed in HEK293F cells.\u003c\/em\u003e\u003c\/p\u003e\n\n\u003c!-- Sources:\n- UniProt: https:\/\/www.uniprot.org\n- NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n- Cusabio: https:\/\/www.cusabio.com\n- Human Protein Atlas: https:\/\/www.proteinatlas.org\n- PubMed: https:\/\/pubmed.ncbi.nlm.nih.gov\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 uL","offer_id":53248423068013,"sku":"CSB-RA820141A0HU-50UL","price":210.0,"currency_code":"USD","in_stock":true},{"title":"100 uL","offer_id":53248496664941,"sku":"CSB-RA820141A0HU-100UL","price":350.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-RA820141A0HU-WB.jpg?v=1776795940","url":"https:\/\/www.ebiohippo.com\/products\/pdx1-recombinant-monoclonal-antibody-bha10576579","provider":"BioHippo","version":"1.0","type":"link"}