{"product_id":"pecr-antibody-peroxisomal-trans-2-enoyl-coa-reductase-bha17136169","title":"PECR Antibody \/ Peroxisomal trans-2-enoyl-CoA reductase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePECR Antibody \/ Peroxisomal trans-2-enoyl-CoA reductase is a anti-PECR Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF) with listed reactivity in Human, Rat. Reported localization: Cytoplasm (Peroxisome).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PECR\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePECR antibody detects Peroxisomal trans-2-enoyl-CoA reductase, an oxidoreductase enzyme that participates in the peroxisomal fatty acid beta-oxidation pathway. The UniProt recommended name is Peroxisomal trans-2-enoyl-CoA reductase (PECR). This enzyme catalyzes the NADPH-dependent reduction of trans-2-enoyl-CoA intermediates to acyl-CoA during the chain elongation and degradation of fatty acids, thereby maintaining lipid homeostasis and energy balance.\u003cbr\u003e\u003cbr\u003eFunctionally, PECR antibody identifies a 303-amino-acid peroxisomal matrix enzyme encoded by the PECR gene, which contributes to the reduction step of unsaturated fatty acid metabolism. PECR participates in both peroxisomal beta-oxidation and fatty acid elongation cycles, converting trans-2-enoyl-CoA substrates into saturated acyl-CoA derivatives for further processing. It plays a critical role in maintaining normal lipid composition within cellular membranes and is essential for the metabolism of very long-chain and polyunsaturated fatty acids.\u003cbr\u003e\u003cbr\u003eThe PECR gene is located on chromosome 2q35 and is expressed in liver, kidney, brain, and skeletal muscle. Its expression is regulated by peroxisome proliferator-activated receptors (PPARs), linking it to lipid metabolism and oxidative stress responses. The enzyme's localization to peroxisomes allows it to cooperate with other beta-oxidation enzymes such as ACOX1 and HSD17B4.\u003cbr\u003e\u003cbr\u003ePathologically, PECR dysfunction or mutation has been associated with metabolic abnormalities and neurodegenerative disease. Variants in PECR can lead to abnormal lipid accumulation and impaired energy metabolism, contributing to disorders such as Refsum-like disease and retinal dystrophy. Research using PECR antibody supports studies in lipid metabolism, peroxisomal function, and oxidative stress regulation.\u003cbr\u003e\u003cbr\u003ePECR antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect peroxisomal enzymes. \u003cbr\u003e\u003cbr\u003eStructurally, Peroxisomal trans-2-enoyl-CoA reductase belongs to the short-chain dehydrogenase\/reductase (SDR) family and contains conserved catalytic motifs (TGxxxGxG and YxxxK) required for NADPH binding and substrate reduction. The enzyme forms homotetramers within peroxisomes and contributes to redox balance via NADPH utilization. This antibody allows investigation of PECR's role in peroxisomal metabolism, energy balance, and cellular redox homeostasis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PECR - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PECR - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PECR - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PECR - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PECR+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047319921005,"sku":"FY13267","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_3e8f745b-e7e7-47b2-9000-f9894c15ce2a.jpg?v=1782237090","url":"https:\/\/www.ebiohippo.com\/products\/pecr-antibody-peroxisomal-trans-2-enoyl-coa-reductase-bha17136169","provider":"BioHippo","version":"1.0","type":"link"}