{"product_id":"pepa-bhb21300268","title":"PEPA","description":"\u003ch3\u003eProduct details\u003c\/h3\u003e \u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eChemical name:\u003c\/strong\u003e 2-(2,6-difluoro-4-(2-(phenylsulfonamido)ethylthio)phenoxy)acetamide.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCAS number:\u003c\/strong\u003e 141286-78-4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular formula:\u003c\/strong\u003e C16H16F2N2O4S2.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt;99.9%\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConcentration:\u003c\/strong\u003e 10 - 250 μM.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilized powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSolubility:\u003c\/strong\u003e 50 mM in DMSO. Centrifuge all product preparations before use (10000 x g for 1 min).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReconstitution:\u003c\/strong\u003e Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShipping:\u003c\/strong\u003e Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e AMPA receptors\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource:\u003c\/strong\u003e Synthetic\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eActivity:\u003c\/strong\u003e PEPA is a selective AMPA receptor agonist (allosteric potentiator)1.\u003c\/li\u003e\n\u003c\/ul\u003e \u003ch3\u003eScientific background\u003c\/h3\u003e \u003cp\u003ePEPA is a flop splice variant and GluR3\/4-prefrerring potentiator of AMPA receptors. PEPA is small chemical compound that enhances AMPA receptor channel activity by modulating receptor kinetics such as desensitization and deactivation. It reportedly enhances synaptic response by selectively acting on AMPA receptors. PEPA (1-200 μM) dose dependently potentiates glutamate-evoked currents in Xenopus oocytes expressing AMPA (GluRA-GluRD). At 200 μM, PEPA could potentiate glutamate responses by 50-fold in oocytes expressing GluRCflop (EC50 ∼50 μM)1. In addition, it potently facilitates extinction learning through activation of AMPA receptors mainly in the medial prefrontal cortex (mPFC)2.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eLead time:\u003c\/strong\u003e 1-2 Business Days\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eCountry of origin:\u003c\/strong\u003e Israel\/IL\u003c\/p\u003e \u003ch3\u003eApplications key\u003c\/h3\u003e \u003cp\u003eApplication key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eBioassay tested:\u003c\/strong\u003e Yes\u003c\/p\u003e","brand":"Alomone Labs","offers":[{"title":"10 mg \/ 1","offer_id":53249711604077,"sku":"P-200-10MG-1","price":117.0,"currency_code":"USD","in_stock":true},{"title":"25 mg \/ 1","offer_id":53311570870637,"sku":"P-200-25MG-1","price":263.0,"currency_code":"USD","in_stock":true},{"title":"5 mg \/ 1","offer_id":53311570903405,"sku":"P-200-5MG-1","price":85.0,"currency_code":"USD","in_stock":true},{"title":"50 mg \/ 1","offer_id":53311570936173,"sku":"P-200-50MG-1","price":476.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/P-200_gr.gif?v=1778544440","url":"https:\/\/www.ebiohippo.com\/products\/pepa-bhb21300268","provider":"BioHippo","version":"1.0","type":"link"}