{"product_id":"pepd-antibody-peptidase-d-bha17135557","title":"PEPD Antibody \/ Peptidase D","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePEPD Antibody \/ Peptidase D is a anti-PEPD Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PEPD\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePEPD antibody detects Peptidase D, a cytosolic metalloprotease involved in the final step of collagen degradation and proline recycling. PEPD hydrolyzes dipeptides containing proline or hydroxyproline at the carboxy terminus, contributing to amino acid salvage and collagen turnover. The PEPD antibody is widely used in metabolism, connective tissue, and enzymology research to study peptide hydrolysis, collagen catabolism, and metabolic disorders.\u003cbr\u003e\u003cbr\u003ePEPD is encoded by the PEPD gene located on human chromosome 19q13.11. The protein is approximately 493 amino acids long and requires manganese as a cofactor for catalytic activity. PEPD functions as a homodimer, with each subunit contributing to the active site. It is expressed in a wide range of tissues, particularly in the liver, kidney, and fibroblasts, where it facilitates collagen metabolism and amino acid homeostasis.\u003cbr\u003e\u003cbr\u003eThe PEPD antibody detects a 54 kilodalton band by western blot and shows cytosolic distribution under immunofluorescence microscopy. PEPD participates in proline recycling for collagen resynthesis, making it essential for connective tissue maintenance and wound healing. Mutations in PEPD cause prolidase deficiency, a rare metabolic disorder characterized by skin ulcers, developmental delay, and recurrent infections due to impaired collagen degradation and imbalanced proline metabolism.\u003cbr\u003e\u003cbr\u003eIn addition to its metabolic role, PEPD modulates cell signaling by acting as an extracellular ligand for epidermal growth factor receptor (EGFR), activating proliferative and survival pathways in response to tissue injury. It also influences immune responses by regulating proline-derived metabolites that affect macrophage activation and oxidative balance.\u003cbr\u003e\u003cbr\u003eBecause PEPD connects collagen metabolism, cell signaling, and tissue repair, it serves as an important target in research on fibrosis, wound healing, and metabolic disorders.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PEPD - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PEPD - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PEPD - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PEPD - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PEPD+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047296000365,"sku":"FY12654","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_33283c61-045d-4a95-b9e1-72d15dc2841c.jpg?v=1782237028","url":"https:\/\/www.ebiohippo.com\/products\/pepd-antibody-peptidase-d-bha17135557","provider":"BioHippo","version":"1.0","type":"link"}