{"product_id":"pepmute-plus-sirna-transfection-reagent-bht2200056","title":"PepMute™ Plus siRNA Transfection Reagent","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eAn upgrade on PepMute™ Reagent (Cat # SL100566), PepMute™ Plus siRNA \u0026amp; DNA Transfection Reagent owes its improvement to pre-screened hydrophobic amino acids crosslinked onto the peptide backbone. Every amine group along that 36–amino-acid backbone has been modified with hydrophobic amino acids, drawing on SignaGen's proprietary pH-Dependent Conformational Change (PDCC) technology. Self-assembly while the transfection complex forms follows from this design, yielding what SignaGen calls a highly versatile and powerful gene delivery platform.\u003c\/p\u003e\n\u003cp\u003eAt 1 nM siRNA, silencing efficiency exceeds 95% across mammalian cell types of many kinds. Single DNA, single siRNA, and co-transfection of DNA together with siRNA have each been validated as efficient and reproducible in various mammalian cells.\u003c\/p\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PepMute_Plus.jpg?v=1790173498\" alt=\"PepMute™ Plus siRNA Transfection Reagent – cartoon illustrating how the reagent was developed\" loading=\"lazy\"\u003e\u003cfigcaption\u003eFigure 1. A cartoon showing PepMute™ Plus siRNA Transfection Reagent was developed\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003esiRNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eNo — General-Purpose Formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C for PepMute™ Plus Reagent and RT for PepMute™ Transfection Buffer (5x). If stored properly, the product is stable for 12 months or longer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePepMute™ Plus Transfection Reagent, 1.0 mL, sufficient for ~1000 reactions based on transfecting 2.5 pmols siRNA in 24-well plate\u003c\/li\u003e\n\u003cli\u003ePepMute™ Plus Transfection Buffer(5x), formulated for maximal transfection efficiency, 8.0 mL\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEffective knockdown (95%) using just 0.5 pmol siRNA (1.0 nM siRNA in a 24-well plate)\u003c\/li\u003e\n\u003cli\u003eFormulated for mammalian cells that are hard to transfect\u003c\/li\u003e\n\u003cli\u003eEasy, single-tube reaction\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PepMute_RL.jpg?v=1790173498\" alt=\"PepMute™ Plus siRNA Transfection Reagent – Renilla luciferase siRNA knockdown in HEK293 vs Dharmafect™ 4 and L2K\" loading=\"lazy\"\u003e\u003cfigcaption\u003eFigure 2. Knockdown efficacy comparison of PepMute™ Plus Transfection Reagent (upper panel) vs. Dharmafect™ 4 siRNA Transfection Reagent (middle panel) and Lipofectamine™ 2000 (lower panel) on HEK293 cells. siRNA targeting renilla luciferase at different final concentrations ranging from 0.5 to 20 nM was co-transfected with the renilla luciferase gene (0.5 µg of pRL-CMV DNA per well) by the above three transfection reagents per manufacturer’s protocols into HEK293 cells growing on a 24-well plate. Renilla luciferase activity was determined 24h after post-transfection with the renilla luciferase determination system (Promega). The luminescence was measured from 5.0 µl of lysate during 10s integration with a luminometer (Beckman Coulter LD 400). Luciferase activity was expressed as light units integrated over 10s (RLU) and normalized per mg of cell protein by using the BCA assay. The error bars represent the standard deviation derived from triplicate experiments. Luciferase-silencing efficiency was calculated relative to untreated cells. While PepMute™ Plus and Dharmafect™ 4 reagents delivered significant gene silencing from 1.0 nM of renilla luciferase siRNA, lipofectamine™ 2000 gave good knockdown only after 30 nM (data not shown).\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PepMute_GFP.jpg?v=1790173499\" alt=\"PepMute™ Plus siRNA Transfection Reagent – GFP siRNA knockdown of co-transfected GFP in HepG2 cells\" loading=\"lazy\"\u003e\u003cfigcaption\u003eFigure 3. PepMute™ Plus Transfection Reagent knocked down GFP gene expression in HepG2 cells. GFP cDNA, pEGFP-N3, was co-transfected with a siRNA targeting the GFP gene (final 5.0 nM, right panel) and a sham siRNA (final 5.0 nM, left panel) into HepG2 cells by PepMute™ Plus Transfection Reagent. GFP fluorescence was visualized 24h post-transfection with a Nikon T2000 fluorescence microscope. Quantitative analysis showed that GFP siRNA at 5.0 nM delivered by PepMute™ Plus Transfection Reagent knocked down 96% co-transfected GFP expression in HepG2 cells.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis siRNA transfection reagent supports gene silencing experiments including RNAi screening, target validation, pathway analysis, and functional genomics studies. Effective delivery of siRNA, miRNA, and other small RNA molecules.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042899087725,"sku":"SL100571-0P1ML","price":40.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042905477485,"sku":"SL100571-0P5ML","price":206.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042905510253,"sku":"SL100571-1P0ML","price":398.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042905543021,"sku":"SL100571-1P0MLX5","price":1890.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PepMute_GFP_27ab3365-8123-412d-8a66-63e5e1f7d77a.jpg?v=1790175948","url":"https:\/\/www.ebiohippo.com\/products\/pepmute-plus-sirna-transfection-reagent-bht2200056","provider":"BioHippo","version":"1.0","type":"link"}