{"product_id":"pepmute-sirna-transfection-reagent-bht2200036","title":"PepMute™ siRNA Transfection Reagent","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eViral cell-penetrating peptides (CPPs) provided the inspiration for PepMute™ siRNA Transfection Reagent, a novel siRNA delivery platform. SignaGen puts its gene silencing at exceptionally high levels — over 95% knockdown at 1 nM siRNA, across a variety of mammalian cell types. The reagent came out of SignaGen's proprietary Peptide Simulation Technology (PST): hundreds of viral CPPs were simulated computationally, then synthesized and screened for how well they delivered genes into diverse mammalian cells.\u003c\/p\u003e\n\u003cp\u003ePepMute™ emerged from that work, validated as an exceptionally effective vector for condensing short (under 100 bp) single- or double-stranded nucleic acids — siRNAs, miRNA mimics, DNA oligonucleotides — and carrying them into a broad spectrum of mammalian cells.\u003c\/p\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PepMute_PC_Simulation.jpg?v=1790173499\" alt=\"PepMute™ siRNA Transfection Reagent – cartoon illustrating development of the reagent by PST\" loading=\"lazy\"\u003e\u003cfigcaption\u003eFigure 1. A cartoon showing PepMute™ siRNA Transfection Reagent was developed by PST\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003esiRNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eNo — General-Purpose Formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C for PepMute™ Reagent and RT for PepMute™ Transfection Buffer (5x). If stored properly, the product is stable for 12 months or longer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePepMute™ Reagent, 1.0 mL, sufficient for ~1,333 reactions based on transfecting 0.5~5.0 pmol siRNA or miRNA mimics in a 24-well plate\u003c\/li\u003e\n\u003cli\u003ePepMute™ Transfection Buffer (5x), formulated for maximal transfection efficiency, 8.0 mL at 5x concentrated stock solution to make 40 mL of working solution\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eExcellent silencing at a final siRNA concentration of 1.0 nM\u003c\/li\u003e\n\u003cli\u003eOver 95% gene silencing across a wide variety of cells\u003c\/li\u003e\n\u003cli\u003eSingle-tube reaction with a simple standard protocol\u003c\/li\u003e\n\u003cli\u003eWorks in the presence of serum and antibiotics\u003c\/li\u003e\n\u003cli\u003eProtocols adapted for HTS\u003c\/li\u003e\n\u003cli\u003eCytotoxicity kept very low\u003c\/li\u003e\n\u003cli\u003eHighly affordable\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PepMute_Comparison_EG5.jpg?v=1790173498\" alt=\"PepMute™ siRNA Transfection Reagent – KIF11 (EG5) siRNA knockdown in HEK293 vs RNAiMAX, INTERFERin, Dharmafect and jetPRIME\" loading=\"lazy\"\u003e\u003cfigcaption\u003eFigure 2. Excellent silencing of endogenously expressed KIF11 (also known as EG5) in HEK293 cells with 1.0 µl of PepMute™ reagent and 0.5 pmol EG5 siRNA per well of 24-well plate. KIF11 (also known as EG5) encodes a motor protein that belongs to the kinesin-like protein family involved in chromosome positioning and bipolar spindle formation during cell mitosis. A reduction in KIF11 levels causes mitotic arrest. PepMute™ reagent effectively delivers EG5 siRNA (final 1.0 nM) to HEK293 cells, leading to more than 80% of “round-up” phenotype of HEK293 cells 48h post transfection over negative control (final 1.0 nM with sham EG5 siRNA) while leading siRNA transfectin reagents, Lipofectamine™ RNAiMAX (RNAiMAX, 1.0 nM EG5 siRNA) \/ INTERFERin (1.0 nM EG5 siRNA) \/ Dharmafect (10.0 nM EG5 siRNA) and jetPRIME (20 nM EG5 siRNA) give average 37%, 23%, 53% and 48% ball-shaped phenotype respectively on HEK293 cells. The phenotype of “rounded-up” 293 cells was visualized (upper panel) and quantified (lower panel) 48h post-transfection with a Nikon microscope.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PepMute_RL2.jpg?v=1790173499\" alt=\"PepMute™ siRNA Transfection Reagent – Renilla luciferase siRNA knockdown in A549 cells vs Dharmafect 4 and RNAiMAX\" loading=\"lazy\"\u003e\u003cfigcaption\u003eFigure 3. Silencing efficiency comparison of PepMute™ Transfection Reagent (upper panel) with Dharmafect 4 (middle panel) and Lipofectamine™ RNAiMAX (RNAiMAX, lower panel) siRNA Transfection Reagents on A549 cells. siRNA targeting renilla luciferase at different final concentrations ranging from 0.5 to 20 nM was co-transfected with the renilla luciferase gene (0.5 µg of pRL-CMV DNA per well) by the above three transfection reagents per manufacturer’s protocols into A549 cells growing on a 24-well plate. Renilla luciferase activity was determined 36h after post co-transfection with the renilla luciferase determination system (Promega). The luminescence was measured from 5.0 µl of lysate during 10s integration with a luminometer (Beckman Coulter LD 400). Luciferase activity was expressed as light units integrated over 10s (RLU) and normalized per mg of cell protein by using the BCA assay. The error bars represent the standard deviation derived from triplicate experiments. Luciferase-silencing efficiency was calculated relative to untreated cells. While PepMute™ and Dharmafect™ 4 reagents delivered significant gene silencing from 1.0 nM of renilla luciferase siRNA, Lipofectamine™ RNAiMAX gave good knockdown only after 20 nM, while enhanced gene expression at low concentrations of siRNA (0.5 and 1.0 nM, respectively) was observed.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PepMute_MCF7.jpg?v=1790173498\" alt=\"PepMute™ siRNA Transfection Reagent – knockdown of stable GFP expression in MCF7 cells with GFP siRNA\" loading=\"lazy\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PepMute_U2OS.jpg?v=1790173498\" alt=\"PepMute™ siRNA Transfection Reagent – knockdown of stable GFP expression in U2OS cells with GFP siRNA\" loading=\"lazy\"\u003e\u003cfigcaption\u003eFigure 4. PepMute™ Transfection Reagent knocked down stable GFP expression in MCF7 cells (upper panel) and U2OS cells (lower panel) by reversely transfecting 5.0 and 1.0 nM GFP siRNA, respectively. Green fluorescence protein (GFP) was stably expressed in MCF7 and U2OS cells. siRNA targeting GFP gene (right panel) and a sham siRNA (left panel) were introduced into MCF7 and U2OS cells with final 5.0 and 1.0 nM, respectively, by reverse transfection with PepMute™ Transfection Reagent. GFP gene silencing was monitored 48h post-transfection by a Nikon fluorescence microscope. Quantitative analysis showed that GFP siRNA at 5.0 and 1.0 nM delivered by PepMute™ siRNA Transfection Reagent knocked down 90% and 95% stably expressed GFP in MCF7 and U2OS cells, respectively.\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eTransfection of siRNA, miRNA mimics, or mRNA\u003c\/li\u003e\n\u003cli\u003eCo-transfection of DNA and siRNA\u003c\/li\u003e\n\u003cli\u003eTransfection of DNA oligos\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042900693357,"sku":"SL100566-0P1ML","price":26.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042904920429,"sku":"SL100566-0P5ML","price":116.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042904953197,"sku":"SL100566-1P0ML","price":218.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042904985965,"sku":"SL100566-1P0MLX5","price":1035.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-PepMute-siRNA-Transfection-Reagent_5b08449c-4358-49a6-9326-c8d8e73ff875.jpg?v=1790175947","url":"https:\/\/www.ebiohippo.com\/products\/pepmute-sirna-transfection-reagent-bht2200036","provider":"BioHippo","version":"1.0","type":"link"}