{"product_id":"pex1-antibody-peroxisome-biogenesis-factor-1-bha17135719","title":"PEX1 Antibody \/ Peroxisome biogenesis factor 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePEX1 Antibody \/ Peroxisome biogenesis factor 1 is a anti-PEX1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PEX1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePEX1 antibody detects Peroxisome biogenesis factor 1, an ATPase essential for the assembly and maintenance of functional peroxisomes. Encoded by the PEX1 gene on chromosome 7q21.2, this cytoplasmic AAA-type ATPase facilitates peroxisomal matrix protein import by recycling the peroxisomal targeting receptor PEX5. PEX1 acts in concert with PEX6 to drive the dislocation and recycling of receptor proteins from the peroxisomal membrane back to the cytosol, ensuring continued import of matrix enzymes required for lipid metabolism and detoxification.\u003cbr\u003e\u003cbr\u003ePEX1 contains two ATPase domains characteristic of the AAA+ protein family and forms a complex with PEX6 that attaches to the peroxisomal membrane via PEX26. This complex functions as an energy-dependent disassembly machine, resetting the import apparatus after cargo delivery. Proper PEX1 activity is essential for peroxisome maintenance and metabolic homeostasis, including beta-oxidation of very long-chain fatty acids and plasmalogen synthesis.\u003cbr\u003e\u003cbr\u003eThe PEX1 antibody is widely used in cell biology, metabolism, and genetic disease research to study peroxisomal biogenesis, import machinery, and metabolic regulation. Western blot analysis identifies a 147 kilodalton band corresponding to PEX1, while immunofluorescence shows punctate cytoplasmic staining consistent with peroxisomal localization. This antibody provides a sensitive tool for investigating peroxisomal function and organelle dynamics.\u003cbr\u003e\u003cbr\u003eMutations in PEX1 are the most common cause of Zellweger spectrum disorders, a group of peroxisomal biogenesis disorders leading to severe metabolic dysfunction. Loss of PEX1 disrupts enzyme import, leading to defective peroxisome assembly and accumulation of toxic lipid intermediates. The PEX1 antibody supports studies of peroxisome biology, organelle biogenesis, and genetic metabolic disease.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PEX1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PEX1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PEX1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PEX1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PEX1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047303242093,"sku":"FY12817","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c7f5a94f-bbee-401b-b47f-1705aacd0732.jpg?v=1782237040","url":"https:\/\/www.ebiohippo.com\/products\/pex1-antibody-peroxisome-biogenesis-factor-1-bha17135719","provider":"BioHippo","version":"1.0","type":"link"}