{"product_id":"pfdn2-antibody-prefoldin-subunit-2-bha17136225","title":"PFDN2 Antibody \/ Prefoldin subunit 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePFDN2 Antibody \/ Prefoldin subunit 2 is a anti-PFDN2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Rat. Reported localization: Nucleus, Cytoplasm, Mitochondria.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PFDN2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePFDN2 antibody detects Prefoldin subunit 2, a cytoplasmic molecular chaperone encoded by the PFDN2 gene on chromosome 1q23.3. PFDN2 is an essential component of the prefoldin complex, a hexameric cochaperone that assists in folding nascent polypeptides and preventing aggregation of cytoskeletal proteins such as actin and tubulin. The prefoldin complex captures newly synthesized, unfolded proteins and transfers them to the group II chaperonin CCT (TCP-1 ring complex) for final folding, ensuring proper assembly of cytoskeletal and structural proteins required for cell function.\u003cbr\u003e\u003cbr\u003eBy stabilizing actin and tubulin subunits, PFDN2 supports cytoskeletal organization, cell division, and intracellular transport. Its role as a chaperone subunit is central to maintaining proteostasis, allowing cells to manage misfolded proteins during stress conditions. PFDN2 expression is ubiquitous and tightly regulated to sustain cytoskeletal dynamics across tissues, particularly in neurons, epithelial cells, and fibroblasts.\u003cbr\u003e\u003cbr\u003eStructurally, PFDN2 forms one arm of the jellyfish-shaped prefoldin complex, which consists of six subunits (PFDN1-6) arranged in a heterohexameric structure. This configuration provides flexibility for binding diverse substrates. PFDN2 contains coiled-coil and beta-hairpin motifs that mediate interactions with unfolded protein substrates, helping maintain their solubility until delivery to the CCT complex. Its conservation across eukaryotes highlights its fundamental role in protein quality control and cellular stability.\u003cbr\u003e\u003cbr\u003ePFDN2 also participates in transcriptional regulation and cellular stress responses, possibly through nuclear interactions under certain conditions. It functions within the broader protein folding and proteostasis network, cooperating with heat shock proteins such as HSP70 and chaperonins to maintain a balanced proteome. Disruption of PFDN2 or other prefoldin subunits leads to cytoskeletal instability, reduced cell proliferation, and heightened sensitivity to proteotoxic stress.\u003cbr\u003e\u003cbr\u003eDysregulation of PFDN2 expression has been linked to several diseases. Reduced prefoldin activity can contribute to neurodegenerative disorders where protein misfolding is pathogenic, such as Parkinson's and Huntington's disease. Overexpression of PFDN2 has been noted in certain cancers, including hepatocellular and colorectal carcinoma, where enhanced folding capacity may support tumor cell growth and resistance to stress. PFDN2 operates in the protein folding and cytoskeletal assembly pathways, underscoring its role in maintaining cell shape and function.\u003cbr\u003e\u003cbr\u003eImmunohistochemical staining using PFDN2 antibody demonstrates cytoplasmic localization consistent with its chaperone activity. The PFDN2 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PFDN2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PFDN2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PFDN2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PFDN2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PFDN2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047322247533,"sku":"FY13323","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_2197ab23-9a12-438c-86e0-3f3e20cc493c.jpg?v=1782237103","url":"https:\/\/www.ebiohippo.com\/products\/pfdn2-antibody-prefoldin-subunit-2-bha17136225","provider":"BioHippo","version":"1.0","type":"link"}