{"product_id":"pfkm-antibody-phosphofructokinase-muscle-type-bha17136008","title":"PFKM Antibody \/ Phosphofructokinase muscle type","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePFKM Antibody \/ Phosphofructokinase muscle type is a anti-PFKM Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PFKM\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, IF, IHC, ICC, WB, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePFKM antibody detects Phosphofructokinase, muscle type, a key glycolytic enzyme that catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. The UniProt recommended name is ATP-dependent 6-phosphofructokinase, muscle type (PFKM). This enzyme controls a rate-limiting step in glycolysis and is a central regulator of glucose metabolism and energy production in muscle tissue.\u003cbr\u003e\u003cbr\u003eFunctionally, PFKM antibody identifies a 780-amino-acid cytosolic enzyme that forms homo- or heterotetramers with other phosphofructokinase isoforms (PFKL and PFKP). PFKM activity is allosterically regulated by ATP, citrate, AMP, and fructose-2,6-bisphosphate, allowing fine-tuned control of glycolytic flux according to cellular energy status. Its catalytic action commits glucose to the glycolytic pathway, linking energy demand with carbohydrate metabolism.\u003cbr\u003e\u003cbr\u003eThe PFKM gene is located on chromosome 12q13.11 and is predominantly expressed in skeletal and cardiac muscle. It plays a vital role in maintaining muscle energy supply during contraction. PFKM expression and activity adapt to metabolic cues, hypoxia, and hormonal regulation, integrating glycolysis with systemic energy homeostasis.\u003cbr\u003e\u003cbr\u003ePathologically, mutations in PFKM cause Glycogen storage disease type VII (Tarui disease), characterized by exercise intolerance, muscle cramps, and myoglobinuria. Deficiency leads to impaired glycolytic ATP production and accumulation of glycogen in muscle fibers. Aberrant PFKM regulation is also associated with metabolic disorders and tumor glycolysis (Warburg effect). Research with PFKM antibody supports studies in metabolism, enzymology, and inherited myopathies.\u003cbr\u003e\u003cbr\u003ePFKM antibody is suitable for western blotting, immunohistochemistry, and enzyme assays to detect muscle phosphofructokinase expression. \u003cbr\u003e\u003cbr\u003eStructurally, Phosphofructokinase, muscle type forms a tetrameric enzyme with alternating catalytic and regulatory domains. Allosteric binding sites modulate its conformational state and enzymatic efficiency. This antibody allows detailed analysis of PFKM's contribution to energy metabolism and metabolic control.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PFKM - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PFKM - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PFKM - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PFKM - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PFKM+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047312253293,"sku":"FY13106","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ce4c78dd-e6fd-4e1a-989f-f07fb0205bf2.jpg?v=1772019398","url":"https:\/\/www.ebiohippo.com\/products\/pfkm-antibody-phosphofructokinase-muscle-type-bha17136008","provider":"BioHippo","version":"1.0","type":"link"}