{"product_id":"pfu-dna-polymerase-bhp15200309","title":"Pfu DNA Polymerase","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eDNA Polymerases\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003ePfu DNA Polymerase is a thermostable protein isolated and purified from recombinant E.coli strains containing Pyrococcus furiosus DNA Polymerase gene, with a molecular weight of about 90 kDa. With 5'→3' polymerase activity and 3'→5' exonuclease activity, it can remove mismatched bases inserted during extension. Pfu DNA Polymerase is a high-fidelity thermostable polymerase. The PCR product is blunt-ended and can be directly linked to the blunt-ended cloning vector, or the end is treated with A and then connected to the TA vector.\u003c\/p\u003e\n\u003cp\u003eThe enzyme is supplied at 5 U\/µL together with 10× Pfu Buffer and ddH₂O for 50 µL PCR reactions; dNTPs are not supplied. The enzyme can amplify single-copy genes from the human genome. A 50 µL reaction uses 0.5 µL of enzyme and 1 µL of dNTP mix and is cycled at 94°C for 3 min, then 30 cycles of 94°C for 30 sec, 50–60°C for 30 sec and 72°C for 30 sec\/kb, with a final 72°C for 5 min.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration\u003c\/th\u003e\n\u003ctd\u003e5 U\/µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUnit definition\u003c\/th\u003e\n\u003ctd\u003e1 unit incorporates 10 nmol deoxynucleotides in 30 minutes at 74°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSource\u003c\/th\u003e\n\u003ctd\u003eRecombinant (E. coli)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eActivities\u003c\/th\u003e\n\u003ctd\u003e5'→3' polymerase and 3'→5' exonuclease (proofreading)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003eGreater than 99% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003eAbout 90 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSupplied buffer\u003c\/th\u003e\n\u003ctd\u003e10× Pfu Buffer (dNTPs not supplied)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTemplate input per 50 µL\u003c\/th\u003e\n\u003ctd\u003eHuman genomic DNA 0.05–0.5 µg; E. coli genomic DNA 10–100 ng; λ DNA 0.5–5 ng; plasmid DNA 0.1–10 ng\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct ends\u003c\/th\u003e\n\u003ctd\u003eBlunt; can be A-tailed for TA vector cloning\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e50 µL\u003c\/th\u003e\n\u003cth\u003e100 µL\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePfu DNA Polymerase (5 U\/µL)\u003c\/td\u003e\n\u003ctd\u003e50 µL\u003c\/td\u003e\n\u003ctd\u003e100 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10× Pfu Buffer\u003c\/td\u003e\n\u003ctd\u003e0.5 mL\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eddH₂O\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003ctd\u003e1 mL × 2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eHigh-fidelity PCR amplification\u003c\/li\u003e\n\u003cli\u003eAmplification of single-copy genes from human genomic DNA\u003c\/li\u003e\n\u003cli\u003eBlunt-end cloning of PCR products\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eAll components are stored at -20°C with a shelf life of two years.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003edNTPs are not supplied and must be added separately; the 50 µL protocol adds 1 µL of dNTP mix, and the working concentration should be confirmed with the manufacturer before use.\u003c\/li\u003e\n\u003cli\u003eAdd Pfu DNA Polymerase after the dNTPs to prevent exonuclease degradation of the dNTPs.\u003c\/li\u003e\n\u003cli\u003eMix the buffer thoroughly before use.\u003c\/li\u003e\n\u003cli\u003eTemplate purity is critical; impurities should not exceed one tenth of the reaction volume.\u003c\/li\u003e\n\u003cli\u003eDilute previously amplified products 1,000–10,000× before re-amplification.\u003c\/li\u003e\n\u003cli\u003eAssemble the reaction components on ice and centrifuge briefly before cycling.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEQ008\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHP15200309\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"100 uL","offer_id":53965434552685,"sku":"EQ008-100UL","price":176.8,"currency_code":"USD","in_stock":true},{"title":"50 uL x 10","offer_id":53965434585453,"sku":"EQ008-50ULX10","price":0.0,"currency_code":"USD","in_stock":true},{"title":"100 uL x 10","offer_id":53965434618221,"sku":"EQ008-100ULX10","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EQ008-a-1.jpg?v=1789416793","url":"https:\/\/www.ebiohippo.com\/products\/pfu-dna-polymerase-bhp15200309","provider":"BioHippo","version":"1.0","type":"link"}