{"product_id":"pgam5-antibody-phosphoglycerate-mutase-family-member-5-bha17136098","title":"PGAM5 Antibody \/ Phosphoglycerate mutase family member 5","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePGAM5 Antibody \/ Phosphoglycerate mutase family member 5 is a anti-PGAM5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Mitochondria.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PGAM5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePGAM5 antibody detects Phosphoglycerate mutase family member 5, a mitochondrial serine\/threonine phosphatase involved in apoptosis, mitophagy, and stress-induced signal transduction. The UniProt recommended name is Phosphoglycerate mutase family member 5 (PGAM5). Despite its name, PGAM5 lacks mutase activity and instead functions as a protein phosphatase that regulates mitochondrial dynamics and cellular stress responses.\u003cbr\u003e\u003cbr\u003eFunctionally, PGAM5 antibody identifies a 289-amino-acid mitochondrial protein localized to the outer mitochondrial membrane. PGAM5 acts downstream of the MAP3K5-ASK1 signaling axis, dephosphorylating substrates that mediate programmed necrosis and mitophagy. It interacts with proteins such as KEAP1, DRP1, and BCL-XL, influencing mitochondrial fission, redox homeostasis, and apoptosis regulation. By modulating DRP1 phosphorylation, PGAM5 facilitates mitochondrial fragmentation during stress-induced cell death and promotes removal of damaged mitochondria through autophagic processes.\u003cbr\u003e\u003cbr\u003eThe PGAM5 gene is located on chromosome 12q24.33 and is expressed in metabolically active tissues, including liver, brain, and skeletal muscle. Through its phosphatase activity, PGAM5 integrates oxidative stress signals with mitochondrial quality control, acting as a central node in determining cell fate under energy or redox imbalance.\u003cbr\u003e\u003cbr\u003ePathologically, dysregulation of PGAM5 contributes to neurodegenerative diseases, ischemia-reperfusion injury, and cancer. In neurons, excessive PGAM5 activation leads to mitochondrial fragmentation and cell death, while reduced expression impairs mitophagy and promotes accumulation of damaged mitochondria. In cancer cells, PGAM5 modulates metabolism and apoptosis sensitivity, linking mitochondrial signaling to tumor progression. Research using PGAM5 antibody supports studies in mitochondrial biology, apoptosis, and stress adaptation pathways.\u003cbr\u003e\u003cbr\u003ePGAM5 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect mitochondrial phosphatases and regulators of cell death. \u003cbr\u003e\u003cbr\u003eStructurally, Phosphoglycerate mutase family member 5 contains a C-terminal catalytic domain with a histidine phosphatase motif (His-Arg-Ser) and an N-terminal transmembrane anchor that tethers it to the outer mitochondrial membrane. The active site structure enables PGAM5 to recognize phosphorylated substrates associated with mitochondrial fission machinery. This antibody facilitates exploration of PGAM5's role in mitochondrial remodeling, necroptosis, and energy metabolism.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PGAM5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PGAM5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PGAM5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PGAM5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PGAM5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047318905197,"sku":"FY13196","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_199d21a2-4be3-4164-8d25-14401037a37b.jpg?v=1782237077","url":"https:\/\/www.ebiohippo.com\/products\/pgam5-antibody-phosphoglycerate-mutase-family-member-5-bha17136098","provider":"BioHippo","version":"1.0","type":"link"}