{"product_id":"pgf2a-prostaglandin-f2-alpha-elisa-kit-bhe15200458","title":"PGF2a(Prostaglandin F2 Alpha) ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003ePGF2a (Prostaglandin F2 Alpha)\u003c\/strong\u003e is a biologically relevant protein marker measured to support mechanistic studies and biomarker discovery (context dependent).\u003c\/p\u003e\u003cp\u003eProtein concentrations can change due to secretion, degradation, cell composition shifts, or post-transcriptional regulation, so ELISA readouts often add information beyond gene expression alone.\u003c\/p\u003e\u003cp\u003eQuantitative measurements help compare groups and time points using standardized curves and can be interpreted alongside phenotype and pathway-specific readouts.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003ePGF2a (Prostaglandin F2 Alpha)\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eGeneral\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PGF2a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PGF2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PGF2a in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: serum, plasma and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 12.5-800 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 3.95 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 2h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52964563812717,"sku":"ELK7850-96T","price":488.8,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52964563845485,"sku":"ELK7850-48T","price":344.5,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52964563878253,"sku":"ELK7850-96TX5","price":2077.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_e19d4924-01f4-46c1-b1ad-d610e87cf456.jpg?v=1771840684","url":"https:\/\/www.ebiohippo.com\/products\/pgf2a-prostaglandin-f2-alpha-elisa-kit-bhe15200458","provider":"BioHippo","version":"1.0","type":"link"}