{"product_id":"phf8-antibody-phd-finger-protein-8-bha17136326","title":"PHF8 Antibody \/ PHD finger protein 8","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePHF8 Antibody \/ PHD finger protein 8 is a anti-PHF8 Rabbit antibody Recombinant Rabbit Monoclonal clone ADAI-16 supplied in Purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PHF8\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone ADAI-16, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePHD finger protein 8 (PHF8) is a histone demethylase that specifically removes methyl groups from lysine residues on histone H3, including H3K9me2 and H3K27me2. By altering chromatin structure, PHF8 regulates transcriptional activation and repression, influencing processes such as cell cycle progression, neuronal development, and differentiation.    \u003cp\u003ePHF8 contains a PHD finger domain that recognizes specific histone modifications, allowing it to target distinct genomic loci. Mutations in PHF8 have been associated with X-linked intellectual disability and craniofacial anomalies. Research on PHF8 provides valuable insights into epigenetic regulation, development, and disease mechanisms. \u003c\/p\u003e\n\u003cp\u003eUsing a high-quality PHF8 antibody enables precise detection in applications such as western blot, immunohistochemistry, and chromatin immunoprecipitation. A PHF8 antibody from\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PHF8 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PHF8 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PHF8 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PHF8 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PHF8+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047325688173,"sku":"RQ8907","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_97d5ce32-0cd6-400d-9d23-df7158c17bd0.jpg?v=1772019444","url":"https:\/\/www.ebiohippo.com\/products\/phf8-antibody-phd-finger-protein-8-bha17136326","provider":"BioHippo","version":"1.0","type":"link"}