{"product_id":"phf8-antibody-plant-homeodomain-finger-protein-8-bha17135488","title":"PHF8 Antibody \/ Plant homeodomain finger protein 8","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePHF8 Antibody \/ Plant homeodomain finger protein 8 is a anti-PHF8 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PHF8\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePHF8 antibody detects Plant homeodomain finger protein 8, a histone demethylase that regulates chromatin structure and transcription by removing methyl groups from lysine residues on histones. PHF8 is a member of the JmjC domain-containing family of histone demethylases and is particularly known for its ability to demethylate mono- and dimethylated histone H3 lysine 9 and lysine 27. Through this enzymatic activity, PHF8 influences gene activation during development, cell cycle progression, and neuronal differentiation. The PHF8 antibody is widely used in epigenetics and developmental biology research to study histone modification, chromatin remodeling, and transcriptional regulation.\u003cbr\u003e\u003cbr\u003ePHF8 is encoded by the PHF8 gene located on human chromosome Xp11.22. The protein is approximately 1,024 amino acids in length and contains two major domains: a plant homeodomain (PHD) finger that recognizes trimethylated histone H3 lysine 4 and a Jumonji C (JmjC) catalytic domain responsible for demethylation. By binding to active chromatin marks via the PHD domain, PHF8 is recruited to promoter regions where it removes repressive histone marks, facilitating transcriptional initiation.\u003cbr\u003e\u003cbr\u003eThe PHF8 antibody detects a 120-140 kilodalton protein by western blot and shows nuclear localization under immunofluorescence microscopy. PHF8 participates in multiple developmental processes, including craniofacial morphogenesis and neural differentiation. In neurons, it regulates genes required for synaptic growth, neurite extension, and neurotransmission. Loss of PHF8 function leads to intellectual disability, cleft lip and palate, and X-linked mental retardation syndromes, emphasizing its critical role in human development.\u003cbr\u003e\u003cbr\u003eAt the molecular level, PHF8 acts as both an epigenetic activator and repressor, depending on context. It associates with transcription factors such as TCF3 and ZNF711 to coordinate developmental gene expression programs. PHF8 also interacts with components of the RNA polymerase II complex, linking histone demethylation to active transcription elongation. In cancer biology, overexpression of PHF8 enhances cell proliferation and epithelial-to-mesenchymal transition, while its depletion suppresses tumor growth by re-establishing chromatin repression.\u003cbr\u003e\u003cbr\u003eBecause of its dual functions in transcriptional activation and chromatin organization, PHF8 serves as an important biomarker for studying epigenetic mechanisms in development and disease.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PHF8 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PHF8 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PHF8 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PHF8 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PHF8+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047293542765,"sku":"FY12585","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_6eedf9d5-9723-4590-a850-1c7b80d20fbd.jpg?v=1782237020","url":"https:\/\/www.ebiohippo.com\/products\/phf8-antibody-plant-homeodomain-finger-protein-8-bha17135488","provider":"BioHippo","version":"1.0","type":"link"}