{"product_id":"phospho-as160-thr642-antibody-tbc1d4-bha17135746","title":"Phospho-AS160 (Thr642) Antibody \/ TBC1D4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-AS160 (Thr642) Antibody \/ TBC1D4 is a anti-AS160 (phospho-Thr642) Rabbit antibody Recombinant Rabbit Monoclonal clone 31T47 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e AS160 (phospho site: Thr642)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31T47, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-AS160 (Thr642) antibody detects TBC1 domain family member 4 when phosphorylated at threonine 642. This protein is encoded by the TBC1D4 gene and is commonly referred to as AS160, Akt substrate of 160 kDa, and insulin-regulated Rab GTPase-activating protein. TBC1D4 functions as a Rab GAP that regulates trafficking of GLUT4 vesicles in response to insulin signaling. Phosphorylation at Thr642 by Akt relieves its inhibitory GAP activity, allowing Rab GTPases to promote translocation of GLUT4 to the plasma membrane, a critical event in glucose uptake by adipocytes and muscle cells.\u003cbr\u003e\u003cbr\u003ePhospho-AS160 (Thr642) antibody is widely applied in metabolism, endocrinology, and diabetes research. Detecting phosphorylation at Thr642 provides a direct readout of insulin-stimulated Akt signaling and GLUT4 trafficking. This makes the antibody valuable for studies of insulin resistance, obesity, and type 2 diabetes. In addition, TBC1D4 is expressed in other tissues, linking it to energy homeostasis beyond classical insulin-responsive cells.\u003cbr\u003e\u003cbr\u003eApplications for Phospho-AS160 (Thr642) antibody include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blot assays detect phosphorylation changes in response to insulin or exercise, immunohistochemistry maps expression in metabolic tissues, and immunofluorescence highlights vesicle trafficking events. These methods allow researchers to connect molecular signaling to physiological glucose uptake.\u003cbr\u003e\u003cbr\u003eDysregulation of TBC1D4 phosphorylation is linked to insulin resistance and diabetes. Reduced phosphorylation impairs GLUT4 trafficking, while mutations in TBC1D4 cause severe postprandial hyperinsulinemia and muscle glycogen storage abnormalities. By applying Phospho-AS160 (Thr642) antibody, scientists can study both common and rare metabolic disorders.\u003cbr\u003e\u003cbr\u003eBeyond metabolism, TBC1D4 regulates vesicle trafficking in immune cells, neurons, and other systems. Phosphorylation-dependent regulation highlights its integration with Akt signaling in multiple contexts.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=AS160 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=AS160 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=AS160 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/AS160 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=AS160+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=AS160 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047303733613,"sku":"FY12844","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d8c5f1b4-090b-48af-80dc-08c0beebef63.jpg?v=1772019352","url":"https:\/\/www.ebiohippo.com\/products\/phospho-as160-thr642-antibody-tbc1d4-bha17135746","provider":"BioHippo","version":"1.0","type":"link"}