{"product_id":"phospho-bcar1-tyr410-antibody-breast-cancer-anti-estrogen-resistance-protein-1-bha17135343","title":"Phospho-BCAR1 (Tyr410) Antibody \/ Breast cancer anti-estrogen resistance protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-BCAR1 (Tyr410) Antibody \/ Breast cancer anti-estrogen resistance protein 1 is a anti-BCAR1 (phospho-Tyr410) Rabbit antibody Recombinant Rabbit Monoclonal clone 32B03 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e BCAR1 (phospho site: Tyr410)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32B03, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-BCAR1 (Tyr410) antibody detects breast cancer anti-estrogen resistance protein 1 when phosphorylated at tyrosine 410. BCAR1, also known as p130Cas, is an adaptor protein encoded by the BCAR1 gene. It localizes to focal adhesions where it links integrins and growth factor receptors to downstream signaling pathways. Phosphorylation at Tyr410 is a critical event that promotes protein protein interactions and assembly of signaling complexes regulating adhesion, migration, and survival.\u003cbr\u003e\u003cbr\u003ePhospho-BCAR1 (Tyr410) antibody is widely used to study integrin signaling, cytoskeletal dynamics, and cancer biology. BCAR1 phosphorylation at focal adhesions enables activation of Src family kinases and downstream effectors such as Rac and JNK. By detecting the phosphorylated form of BCAR1, this antibody provides insight into how cells sense and respond to extracellular matrix cues.\u003cbr\u003e\u003cbr\u003eThe antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, Phospho-BCAR1 (Tyr410) antibody detects phosphorylated isoforms distinct from non phosphorylated protein. Immunohistochemistry maps phosphorylation events in tissue samples, while immunofluorescence reveals spatial localization of activated BCAR1 at focal adhesions. These approaches allow researchers to monitor cell adhesion signaling with high precision.\u003cbr\u003e\u003cbr\u003eBCAR1 phosphorylation has been linked to oncogenesis and therapy resistance. Elevated phospho BCAR1 levels are associated with enhanced tumor invasion and poor prognosis in breast and other cancers. By using Phospho-BCAR1 (Tyr410) antibody, scientists can evaluate how dysregulated adhesion signaling contributes to malignancy and whether Tyr410 phosphorylation serves as a biomarker of tumor progression.\u003cbr\u003e\u003cbr\u003eBeyond oncology, BCAR1 phosphorylation plays roles in cardiovascular and immune systems. It regulates endothelial cell migration during angiogenesis and contributes to immune cell trafficking. In developmental contexts, BCAR1 activity is essential for morphogenesis and tissue patterning. The phospho specific antibody enables detailed investigation of these roles across models.\u003cbr\u003e\u003cbr\u003ePhospho-BCAR1 (Tyr410) antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=BCAR1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=BCAR1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=BCAR1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/BCAR1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=BCAR1+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=BCAR1 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047288136045,"sku":"FY12440","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_3c632c00-f822-42c9-bd26-7c3ace7d1930.jpg?v=1772019287","url":"https:\/\/www.ebiohippo.com\/products\/phospho-bcar1-tyr410-antibody-breast-cancer-anti-estrogen-resistance-protein-1-bha17135343","provider":"BioHippo","version":"1.0","type":"link"}